Gene Technology

Gene therapy through time

100

The Year In which Jesse Gelsinger case happened 

1999

200

What was the vector used in Jesse Gelsinger's case of OTC

Adenovirus Vector

300

Name of the Person who died after trials for rheumatoid arthritis 

Jolee Mohr

400

The disease associated with Bubble Boy

SCID ( Severe Combined Immunodeficiency)

500

Name of the two genes from which either one can be faulty causing SCID disease

ADA and IL2RG

Gel ElectroPhoresis

100

Term used for small depression/ holes made in the gel

Wells

200

Which tool is used to transfer small liquid solutions of DNA samples from test tubes into gel

Micropipette

300

Name of the gel used if we have to seperate protein fragments

_{Polyacrylamide gel}

400

What is the charge on DNA molecule and why does DNA have this charge?

Negative and Phosphate groups in nucleotides make the DNA negatively charged

500

What is the specific term used for adding or transferring DNA fragments into the depressions

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CRISPR

100

What is the origin of CRISPR? 

E.Coli Bacteria

200

component of the bacterial DNA that protects the DNA itself from being cleaved by the restriction endonuclease

Methyl Groups

300

What does CRISPR stand for?

Clustered Regularly Interspaced Short Palindromic Repeats

400

What is the standard length of CRISPR SEQUENCES

20 nucleotides

500

What is the name of the restriction enzyme used in CRISPR technology

^{Cas 9 endonuclease}

PCR

100

State the Full form of PCR

Polymerase Chain Reaction

200

What is the term for a short sequence of Nucleotides that guides DNA polymerase to build new strands of DNA

Primer

300

Temperature used in Denaturation

Around 90 to 95 degrees Celsius 

400

Name of the specific  DNA Polymerase used in PCR 

Taq Polymerase

500

Name of the process that occurs in PCR at a temperature of around 65 to 68 degrees Celsius 

Annealing 

Overall gene tech

100

GFP ( Green Flourescent Protein) is Synthesized by enzymes found in?

Jellyfish

200

There are three commonly used vectors: Plasmids, Viruses and?

^Liposomes

300

What is the full form of VNTR

^{Variable Number Tandem Repeats}

400

Name the enzyme used to Synthesize single strand of DNA from RNA strand

Reverse Transcriptase

500

Name the bacteria from which The specific polymerase enzyme is extracted for a process of PCR

Thermus Aquaticus