How it Works
Definitions
DNA BASICS
Complex Lab Procedures
Regents Review
100
The helpful gene extracted from a donor organism in genetic engineering. Such as the gene for insulin.
What is the gene of interest? Or the desired gene?
100
The study of how ethically different some biological processes are is called this.
What is bioethics?
100
The number of strands human DNA has.
What are two?
100
An absent or faulty gene is replaced by a working gene.
What is Gene Therapy?
100
46
C
200
The matching ends of DNA sequences created by restriction enzymes
What are sticky ends?
200
DNA that has other genes inserted into it.
What is recombinant DNA?
200
A, C, T, and G are examples of these.
What are nitrogenous bases?
200
The clear suspect who committed the crime... 
Who is John?
200
54
A
300
A circular, single-stranded piece of DNA.
What is a plasmid?
300
These are used for cutting pieces of DNA and plasmids open, creating sticky ends.
What are restriction enzymes?
300
If you accidentally used two different ones of these, genetic engineering would not work because the sticky ends won't match.
What are restrictions enzymes?
300
1.) take a somatic cell from the desired animal 2.) take an unfertilized egg from the egg donor 3.) enucleate (remove the nucleus) from the egg 4.) put the somatic nucleus from the animal to be cloned to the empty egg cell 5.) allow the egg cell to undergo mitosis 6.) insert the embryo into the surrogate 7.) surrogate produces the baby
What are the steps in cloning?
300
57
B
400
E-coli + AmpR vs. E-coli - AmpR
What is bacteria transformation?
400
Something that delivers genetic information to the cell.
What is a cloning vector?
400
Plasmids occur naturally in certain types of this.
What are bacteria?
400
More unique and valuable than fingerprint, this is key evidence to differentiate key suspects in a crime scene.
What is a DNA fingerprint?
400
106
C
500
The receiving organism after it's its DNA has been changed/manipulated.
What is a transgenic organism? Or what is a genetically modified organism (GMO) ?
500
The "glue" that puts sticky ends back together
What is DNA ligase?
500
Nuclear Transplantation is the key technique to this genetic engineering process
What is cloning an organism?
500
1. cut the DNA with a restriction enzyme, 2. plug the DNA into the wells, 3. apply a positive charge at one end and a negative charge at the other end, 4. the DNA bands fragment and travel toward the negative charge, separating according to size
What are the steps in performing a gel electrophoresis?
500
39
A