Enzymes
Strawberry Extraction
pGLO Lab
PTC Taster Lab
Equipment
100
The enzyme which adds new nucleotides to single-stranded DNA. 

What is DNA Polymerase? 

100

Where was the DNA in the strawberries located? 

What is the nucleus?

100

The antibiotic used to select our transformed bacteria

What is ampicillin?

100

The function of Chelex

What is "to separate DNA from other cellular materials?"
100

The best micropipette used to aliquot 2 uL. 

What is a P10?

200

The enzyme which cuts DNA at specific locations.

What is a restriction enzyme? 

200
The membranes of the strawberry cells were broken down by this chemical: ___

What is soap?

200

The medicines that can be made with this process

Insulin, HGH, EPO, monoclonal antibodies, RNA vaccines, etc.

200

The name of the DNA Polymerase used in our experiment.

What is Taq Polymerase? 

200

The device used to bring fluids down to the bottom of a tube by rapid spinning, creating an artificial gravity field.

What is a centrifuge?

300

Which enzyme gene sequence was added in the plasmid that was transferred to our bacteria?

What is beta-lactamase?
300
What was added to precipitate the DNA from the solution?

What is isopropyl alcohol?

300

Extragenomic pieces of DNA commonly found in bacteria.

What are plasmids? 

300
The full name of the process used to amplify our DNA.

What is Polymerase Chain Reaction? 

300

The device used to run a current through a gel during gel electrophoresis.

What is a gel box? 
400

The enzyme which creates RNA for protein synthesis. 

What is RNA Polymerase? 
400

The function of the NaCl salt added during the DNA extracted is: _____

What is neutralizing the negative charge of DNA? 

400

The practices and procedures to prevent contamination from pathogens or germs.

What is aseptic technique?

400

The reason a higher percentage gel is used in this lab.

What is "to separate lower molecular weight DNA samples?" 

400

The lowest accurate reading on a P10?

What is 1 uL?

500
The enzyme which adds a temporary sequence of primers to DNA during DNA replication. 

What is Primase? 

500

The length of the DNA extracted from our strawberries.

840,000,000 miles

500
The expected result of our final plates.

LB: Both pGLO and control, both non-glowing

LB+amp: pGLO, glowing. 

500

The full name of the taste receptor we were studying

What is phenylthiocarbamide?
500

The device used to perform PCR. 

What is a thermocycler?