HGP/Cloning
Technology
Recombinant DNA
Examples
Misc
100
Collection of all of an organism's genes
What is a genome?
100
Definition of Gel Electrophoresis.
What is the process use to separate fragments based on size and charge?
100
Definition of recombinant DNA.
What is a combination of DNA from two different organisms (i.e. frog and bacteria)?
100
Process of breeding individuals by choosing mates to try and have a certain set of traits in offspring.
What is selective breeding?
100
Definition of genetic engineering
What is manipulating genes for practical purposes?
200
Dolly is important in cloning history for this reason
What is she was cloned from adult cell successfully?
200
Uses for gel electrophoresis.
What are criminal cases, paternity cases, identifying a gene of interest?
200
Two enzymes used in producing recombinant DNA.
What are DNA ligase and restriction enzymes?
200
Ways (2) in which plants/animals have advanced due to genetic enginneering.
What are resisted bruising and hormones in milk?
200
Definition of a plasmid
What is a circular piece of bacterial DNA that can be used as a vector?
300
These are the two goals of the Human Genome Project.
What is mapping and sequencing the location of all genes.
300
Ways in which fragments are separated in electrophoresis.
What are charge and size?
300
Product of restriction enzymes
What are sticky ends?
300
First step in genetically engineering insulin
What is identify the healthy insulin gene and cut it out?
300
Term used to describe a free-living organism with foreign DNA inside it's cells.
What is transgenic?
400
The number of genes in the human genome.
What is 25,000-35,000?
400
Way in which you can read evidence using gel electrophoresis.
What is perfect match in criminal cases, 50% match in paternity cases, and probe identification with gene of interest?
400
Examples of restriction enzymes
What are EcoR1, HindIII, BamHI, etc.?
400
State 3/4 genetically engineered medicines that we discussed.
What are insulin, HGH, anticoagulants, and factor VIII?
400
Introduction of a dead or weakened virus to help an organism create immunity
What is vaccine?
500
Definition of gene therapy.
What is the introduction of a healthy gene into a cell containing a faulty gene?
500
Every individual has unique DNA (except for identical twins). This is the basis for _________.
What is DNA fingerprinting?
500
The steps of producing recombinant DNA.
What are cutting DNA, making recombinant DNA, cloning recombinant DNA, and screening?
500
Benefits of genetically engineering medications
What are it is faster, cheaper, and there is no risk of infection/disease?
500
Explain the process of screening using the amp resistance example.
LONG EXPLANATION