DNA (1)
Which two modifications to the genome increase gene expression?
Increased acetylation, decreased methylation
What is the eukaryotic equivalent to DNA Pol III?
DNA Pol α, β, ε
What is formed when RNA Pol II binds with a general transcription factor (ie TFIID)?
Pre-initiation complex (PIC)
Which molecule binds to the 5' cap to recruit the 40S subunit?
eIF4F - eukaryotic initiation factor
What are the modulators of PFK?
+: AMP
-: citrate, high concentration ATP
Which prokaryotic enzyme repairs T-T covalent links?
DNA photolyase
What are the nucleotides used in Sanger sequencing?
Which subunit leads to the binding of RNA Pol (prokaryotes) to specific locations?
σ
How do siRNAs differ from miRNAs?
siRNAs are synthetic
What is the Warburg Effect?
The high glycolytic rate of cancer cells despite abundance of oxygen
What is the telomere tandem repeat sequence?
TTAGGG
What chemical added to DNA makes it fluorescent under UV light? (also a carcinogen)
ethidium bromide
What does CREB stand for? (It is a transcription factor)
cAMP response element binding protein
What are the subunits of the spliceosome?
U1, U2, U4, U5, U5
snRNPs - small nuclear ribonucleoproteins
Which ion's equilibrium potential is the closest to the resting membrane potential?
K+
What are the two repair mechanisms for double stranded nicks?
NHEJ - Non-homologous End Joining
HR - Homologous Repair
What is the peak absorption wavelength of DNA? (UV Spectrum)
260 nm
What do dicistronic assays look for?
IRES - internal ribosome entry sites
What is the splice site sequence?
What domain binds to phosphorylated tyrosine residues of the EGF receptor?
SH2
What are the histone subunits named?
H2a, H2b, H3, H4
What is the most common form of DNA helix?
B helix (other types are A, and Z)
Which molecule binds to the A site of the ribosome upon the reading of a stop codon?
Release Factor
How many As does poly(A) polymerase add to an mRNA?
~250
What is the Michaelis Menten Equation?
Please tell me u know this