DNA Adducts 1
Repair mechanism for a base
What is base excision repair?
This enzyme the deaminated cytosine base.
What is Ung - Uracil Glycosylase
The purpose of the Ames test.
What is to show if a molecule is mutagen?
Amino acids are made from..
What is a biochemical pathway?
The number of hours I should spend studying genetics
What is two hours outside of class for every hour inside of class?
These tandem bases are covalently bonded together.
What are Thymidine dimers?
The site remaining after uracil is removed
What is the AP-site?
Introducing a mistake in DNA
What is a Forward Mutation?
Genes that code for amino acids
What is they don't exist?
When should I be studying for genetics
What is every single day?
Removal of an amino goup from a molecule
What is deamination
The enzyme that removes the remaining backbone from the AP-Site
What is APE --> AP Endonuclease
Correcting a mutation back to the wild-type
What is a reverse mutation?
Just because something is a mutagen
What is doesn't mean its a carcinogen.
To ensure that I get all the information presented in lecture.
What is record the lectures?
Deamination of cytosine.
What is Uracil?
The enzyme that fills the gap (where the nucleotide used to be) in eukaryotes.
What is DNA Pol Beta?
Ames used these particular bacteria to conduct a reversion assay.
What's a his- auxotophic bacteria (Salmonella)?
Added to check if metabolite of a POTENTIAL mutagen was mutagenic.
What is rat liver?
Studying on my own isn't working.
What is I should form a study group or continue to come to SI?
Deamination of Adenine
What is Hypoxanthine?
A type of helicase protein that will open up DNA and excise thymidine dimers from DNA
What is uvR/uvRD proteins?
Ames plating his- salmonella and no mutagen acted as a...
What is a control for the experiment (base line)?
Ames plating his- salmonella and rat liver with no mutagen acted as a....
What is a control in the experiment (base line).
The best place I should go if I need help/guidance.
Where is to Dr. Currs office hours?