Topic 12
Topic 13
Topic 14
Potluck
100

This type of molecule creates condensates through multiple weak and fluctuating binding interactions among themselves. In addition, they recruit specific proteins and nucleic acids into the condensate as client macromolecules.

What are scaffold macromolecules?

100

Unstructured domains of the nuclear pore complex contain numerous repeats of this motif, whose weak affinity for each other creates a gel-like mesh.

What are phenylalanineglycine (FG) motifs?

100

Formation of COPI and clatherin coated vesicles is assisted by this.

What are Arf proteins?

100

One will typically have one or more cis-double bonds present (it’s unsaturated) while the other does not (it’s saturated).

What are the different tail structures of phosphoglyceride? 

200

Tight binding by this hsp70 like chaperone protein prevents the protein chain from sliding backwards during translocation in the ER.

What is BiP?

200

This type of localization signal can be located almost anywhere in the amino acid sequence and are thought to form loops or patches on the protein surface.

What is nuclear localization signal (NLS)?

200

Indicator for vesicles moving into the cell, and indicator for vesicles moving out of the cell.

What are the retrograde and the secretory/anterograde pathways?

200

Two different secondary antibodies are used through labeling with different fluorescent dyes.

What is indirect immunodetection?

300

This type of ER membrane transports vesicles carrying newly synthesized proteins and lipids bud off for transport to the Golgi apparatus.

What is transitional ER (type of smooth ER)?

300

Glycosphingolipids and sphingomyelin are produced on this side of the bilayer leaflet.

What is non-cytosolic?

300

Calnexin, Calreculin and Bip are involved in the folding and maturation of this molecule.

What is IgG?

300

Inhibition of gene expression in bacteria by premature termination of transcription.

What is transcriptional attenuation?

400

In a signal-recognition particle’s large hydrophobic sequence binding site, this amino acid is enriched.

What is methionine?

400

In the mitochondrial intermembrane space, cysteines are oxidized to disulfide bonds by this enzyme.

What is Mia40?

400

The three amino-acid sequence for N-linked glycosylation. 

What is NXT, where N is asparagine, X is any amino acid but proline, and T is either serine or threonine?

400

Glycoproteins have their attached sugars modified at this location.

What is the median domain of the golgi

500

This ER resident protein catalyzes the oxidation of free sulfhydryl (SH) groups on cysteines to form disulfide (S-S) bonds.

What is protein disulfide isomerase (PDI)?

500

Peroxisomal protein import is driven by ATP hydrolysis and utilizes this family of proteins.

What are peroxins (Pex)?

500

When vesicles are moved into a cell and deposited on the other side of the cell (often used for moving IgG).

What is transcytosis?

500

Dosage compensation protein that associates with X-chromosomes and recruits the dosage-compensation complex.

What is Sdc2 and what does it do?