This type of molecule creates condensates through multiple weak and fluctuating binding interactions among themselves. In addition, they recruit specific proteins and nucleic acids into the condensate as client macromolecules.
What are scaffold macromolecules?
Unstructured domains of the nuclear pore complex contain numerous repeats of this motif, whose weak affinity for each other creates a gel-like mesh.
What are phenylalanineglycine (FG) motifs?
Formation of COPI and clatherin coated vesicles is assisted by this.
What are Arf proteins?
One will typically have one or more cis-double bonds present (it’s unsaturated) while the other does not (it’s saturated).
What are the different tail structures of phosphoglyceride?
Tight binding by this hsp70 like chaperone protein prevents the protein chain from sliding backwards during translocation in the ER.
What is BiP?
This type of localization signal can be located almost anywhere in the amino acid sequence and are thought to form loops or patches on the protein surface.
What is nuclear localization signal (NLS)?
Indicator for vesicles moving into the cell, and indicator for vesicles moving out of the cell.
What are the retrograde and the secretory/anterograde pathways?
Two different secondary antibodies are used through labeling with different fluorescent dyes.
What is indirect immunodetection?
This type of ER membrane transports vesicles carrying newly synthesized proteins and lipids bud off for transport to the Golgi apparatus.
What is transitional ER (type of smooth ER)?
Glycosphingolipids and sphingomyelin are produced on this side of the bilayer leaflet.
What is non-cytosolic?
Calnexin, Calreculin and Bip are involved in the folding and maturation of this molecule.
What is IgG?
Inhibition of gene expression in bacteria by premature termination of transcription.
What is transcriptional attenuation?
In a signal-recognition particle’s large hydrophobic sequence binding site, this amino acid is enriched.
What is methionine?
In the mitochondrial intermembrane space, cysteines are oxidized to disulfide bonds by this enzyme.
What is Mia40?
The three amino-acid sequence for N-linked glycosylation.
What is NXT, where N is asparagine, X is any amino acid but proline, and T is either serine or threonine?
Glycoproteins have their attached sugars modified at this location.
What is the median domain of the golgi
This ER resident protein catalyzes the oxidation of free sulfhydryl (SH) groups on cysteines to form disulfide (S-S) bonds.
What is protein disulfide isomerase (PDI)?
Peroxisomal protein import is driven by ATP hydrolysis and utilizes this family of proteins.
What are peroxins (Pex)?
When vesicles are moved into a cell and deposited on the other side of the cell (often used for moving IgG).
What is transcytosis?
Dosage compensation protein that associates with X-chromosomes and recruits the dosage-compensation complex.
What is Sdc2 and what does it do?