Bacteria Basics
Bacterial Colonies
Contamination & Technique
Colony Morphology
Counting Colonies
100

These organisms are microscopic, are self sufficient and made of a single cell.

Bacteria

100

A visible group of bacterial cells growing together on agar

bacterial colony

100

This type of microorganism is introduced accidentally into an investigation.

unwanted microorganisms

100

This descriptor refers to the basic form of a colony.

Shape

100

The abbreviation CFU stands for this.

Colony Forming Unit

200

Bacteria can be either beneficial or ...

Harmful

200

Most colonies grow on this nutrient medium

agar

200

This laboratory technique helps prevent contamination

aseptic technique

200

This descriptor describes the edge of a colony.

Margin

200

Each distinct colony counted is considered one of these.

colony forming unit (CFU)

300

Name one place where bacteria can be found.

soil, water, air, or inside living organisms

300

Colonies form when bacteria do this repeatedly

reproduction/multiplication/division

300

Contamination can make experimental results become this

unreliable

300

This descriptor describes the transparency of a colony.

Opacity

300

A plate should be placed on this type of surface when counting colonies.

well-lit surface

400

Bacteria belong to this level of organisation (in the body).

cell (single-celled organism)

400

Colonies are made of millions of genetically ______ cells

identical
400

Name three methods for preventing contamination

Wear a mask

Wear a lab coat

Sterilise instruments

Only lift the lid of the agar plate slightly

Don't touch the sampling end of the swab


400

The side-view appearance of a colony is called this.

Elevation

400

This tool can be used to ensure colonies are not counted twice.

colony counter or marker

500

Explain one beneficial role bacteria can have.

decomposition, digestion, nutrient cycling, food production

500

Colonies often start from _____ bacterium

one

500

Give one reason why contamination is a major problem in microbiological investigations.

  • Introduces unwanted microorganisms.
  • Makes results unreliable.
  • Makes identification difficult.
  • Affects validity of the investigation.
500

Which characteristic would be describe whether a colony is rough, wrinkled or smooth?

surface

500

A plate contains 33 visible colonies. How many CFU are recorded?

33 CFU