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Sample Collection/Handling
Erythropoeisis
PCV/TP
Blood smear
RBC morphology
100
The anticoagulant of choice for morphology
What is EDTA?
100
Where erythropoeisis occurs
What is bone marrow?
100
Reference ranges for canine and feline PCV.
What is canine- 37-55%, feline- 30-45%
100
Process of staining a slide
What is 10-20 dips in each solution, #1 fixer, #2 pink, #3 purple, rinse back, dry
100
Normal shape of erythrocyte
What is biconcave disc?
200
Tube used for chemistry tests
What is RTT, SST?
200
Hormone that stimulates erythropoeisis
What is erythropoietin?
200
Three components of a centrifuged hematocrit tube, top to bottom
What is plasma/serum, buffy coat, rbc's?
200
3 areas of a smear
What is body, monolayer, feathered edge
200
irregularly shaped margins/projections from the cell wall
What is acanthocyte?
300
Anticoagulant that can be used for chemistry tests
What is lithium heparin?
300
Lifespan of canine and feline erythrocytes
What is canine-120 days, feline-70 days
300
Instrument used to measure total protein
What is refractometer?
300
Where morphology is examined
What is monolayer?
300
Variation in cell shape
What is poikilocytosis?
400
Why you should not freeze whole blood
What is hemolysis?
400
3 rules for aging erythrocytes (what changes as they mature)
What is less nucleus, pink, smaller in size?
400
Calculation for hemoglobin when given PCV
What is PCV/3?
400
Change angle in this way if smear is too short.
What is decrease?
400
Variation in size of cells
What is anisocytosis?
500
Tube used for coagulation testing
What is light blue/sodium citrate?
500
6 stages of erythropoeisis from immature to mature
What is rubriblast, prorubricyte, rubricyte, metarubricyte, polychromatophil, erythrocyte
500
Decreased PCV can indicate
What is anemia?
500
Drop size and spreader angle for an anemic smear.
What is smaller, increase?
500
Erythrocytes with small, dark staining, round, nuclear fragments
What is Howell-jolly bodies?