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100
Shortest verse in the Bible - Reference & Verse.
What is “Jesus wept?”  John 11:35
100
The factors of 25.
What are 1, 5, and 25?
100
Earth's only natural satellite.
What is the moon?
100

The mark used to indicate possession and contractions.

What is an apostrophe?
100
The rest that has a duration of 2 beats.
What is a half rest?
200
I am Moses father-in-law.
Who is Jethro or Reuel?
200
The cube root of 27.
What is 3?
200
This chemical makes plants green.
What is chlorophyll?
200

The part of speech that modifies verbs, adjectives and itself and can be relocated in a sentence much more easily than other words.

What is an adverb?
200
The time signature known as waltz time.

What is    3
               4?

300
How Paul acquired his Roman citizenship.
How is, by birth?
300
The acronym for the order of operations.  Bonus Points.
What is PEMDAS?
300
The Periodic Table category that holds nonreactive gasses.
What are the Noble Gasses?
300
a, an, the
What is an article?
300
The Classical composer who went deaf.
Who is Beethoven?
400
In Revelation, Jesus is described as the first and last letters of the Greek language.
What is Alpha and Omega?
400
A line that extends into infinity in one direction.
What is a ray?
400
The number of chambers in the human heart.
What is 4?
400
42 (bonus points)
What is the meaning of life?
400
The number of notes in an octave?
What is eight?
500
Number of books in the Bible.
What are 27 and 39 or 66 total?
500

a2 + b2 = c2

What is the Pythagorean Theorem?
500
Evaporation, condensation and precipitation.
What is the water cycle?
500
Shakespeare’s home town.
Where is Stratford-on-Avon?
500
The original name for a piano.
What is pianoforte?