Safety
This class of fire involves wood, paper, or plastic
What is a class A fire?
This is when enzymes inside cells start digesting and destroying the cells.
What is autolysis?
What is formaldehyde/formalin
A flotation bath should be maintained this many degrees below the melting point of paraffin
what is 5-10% below
This simple sugar cannot be demonstrated in tissue sections because it is soluble in aqueous solutions
What is glucose
This class of fire involves flammable gases or liquids
What is a Class B fire
This is tissue breakdown caused by bacteria
What is putrefaction
This fixative is most frequently used for electron microscopy but penetrates slowly and poorly
What is glutaraldehyde
Paraffin should be kept this many degrees above its melting point
What is 2-4 degrees Celsius
This group includes glycogen, starch, and cellulose
What are neutral polysaccharides
This class of fire involves electrical equipment and requires non-conductive media
What is a Class C fire?
This is a change in the 3D structure of macromolecules, especially proteins.
What is denaturation?
This fixative renders lipids insoluble and is used for electron microscopy
What is osmium tetroxide
Prolonged time in clearing agents can make tissue this texture
What is hard and brittle
These carbohydrarte stains are listed repeatedly in the notes: PAS, Alcian blue, mucicarmine and this 'iron stain'
What is colloidal iron
This formaldehyde exposure standard is .75ppm over 8 hours.
What is the Time Weighted Average, or TWA
The minimum fixative-to-tissue volume ratio listed in the notes.
This picric acid-based fixative is comonly used for GI biopsies and gives crisper nuclei than 10% NBF
What is Bouin's solution
This ___ can occur from the flotation bath and is described in the notes as "parched earth"
What is a flotation bath artifact
This acid mucopolysaccharide is found in connective tissue and umbilical cord
What is hyaluronic acid
This formaldehyde exposure standard is 2ppm over 15 minutes
What is the Short Term Exposure Limit, or STEL?
Formalin does this quickly, but fixes tissue slowly.
What is penetrates tissue?
This fixative is used for chromaffin granules in the adrenal medulla and diagnosis of pheochromocytoma, unless IHC is being performed.
What is Orth solution
As paraffin melting point increases, paraffin becomes harder, supports better, and ribboning becomes this?
What is harder/more difficult?
This sulfated acid mucopolysaccharide is found in mast cells and the intima of arteries
What is heparin?