HLA Jeopardy Round 1

DNA Extraction & PCR

100

Pure DNA has a ratio of approximately 

1.8-2.0

200

_________digests cellular proteins released following lysis. 

Proteinase K protease

300

Which of the following is the preferred sample for DNA extraction?

a. Whole blood without anticoagulants 

b. Red blood cells 

c. Heparinized serum 

d. Platelets 

e. Whole blood with ACD anticoagulant  

e. Whole blood with ACD anticoagulant  

400

The absolute A260 and A280 values and their ratio for a DNA sample provide what information?

RNA/Protein contamination of DNA

500

In a reverse SSO assay, the genomic DNA is often incubated with sodium hydroxide before application to the membrane. Why?

To denature the DNA

HLA Basics

100

The HLA complex is a cluster of linked genes located on the __________ of Chromosome 6.

Short arm

200

HLA Class I are ____,____, ____ and HLA Class II are ___,___,____

Class I: A, B, C

Class II: DR, DQ, DP

300

______ are the building blocks that are incorporated into the DNA chain during PCR

dNTPs

400

The Donor blood type ____ is compatible with all recipient blood types (A, B, O, AB)

Donor ABO- O

500

It is acceptable for a red top to be collected from a patient who is at that moment receiving a dialysis treatment

false

Terminology

100

HLA

Human Leukocyte Antigen

200

MHC

Major Histocompatibility complex

300

WHO

World Health Organization

400

CREG

Cross-reactive epitope groups

family of HLA antigens that share some, but not all, of the polymorphisms.  For Solid Organ transplant, CREG matches are less likely to be immunogenic because of the shared epitopes (the amino acid motif which is recognized by an antibody)

500

PRA

Panel Reactive Antibody; 

  Indicates the probability of a Positive XM (percentage   of donor pool that is predicted to be positive)

CHT

100

The use of pronase in a flow crossmatch assists in removing non-specific binding due to Fc receptors, as well as

Removing CD20 from the cell surface

200

Ficoll-Hypaque separates lymphocytes based upon what inherent property?

Density

300

High resolution Class I and Class II typing is most important in which of the following situations?

a. Living-related kidney transplantation with high PRA 

b. ABO-mismatched liver transplant 

c. Unrelated hematopoietic stem cell transplantation

d. Autologous hematopoietic stem cell transplantation 

e. Two haplotype matched living related hematopoietic stem cell transplantation  

c. Unrelated hematopoietic stem cell transplantation

400

The structural components of a nucleotide are…

Base, Sugar, Phosphate

500

Molecular typing usually uses genomic DNA as a starting material. If RNA is converted into cDNA for typing, what feature is missing that may require adaptation of methodology from genomic DNA typing? a. Base m

Introns

Regulatory, Quality & Safety

100

PPE stands for

Personal Protective Equipment

200

HLA is inspected and accredited by which agencies

ASHI & CAP

also NYS

300

HLA technologists are required to complete ____hours of Continuing education/year or 1.2 CEC's 

12

400

NAME 3 of the 6 elements a person is evaluated on to determine competency in a test system

Direct observation of routine test performance

Direct observation of instrument maintenance and function

PT or blind sample

problem solving skills and knowledge

Review of records, QC, preventative maintenance and worksheets

Monitoring entry of information into LIS

500

Which of the following activities should be performed in the post-PCR area?

a. Amplification, probing, high stringency washes 

b. DNA extraction, low stringency wash, probing 

c. Gel electrophoresis, probing, primer aliquoting 

d. Amplification, probing, primer aliquoting 

e. DNA extraction, high stringency wash, primer synthesis  

a. Amplification, probing, high stringency washes