Fingerprint Fundamentals
Legacy RFLP
Modern STR Analysis
Identifying the Romanovs
Crime Scene & CODIS Standards
100

This is the most common fingerprint pattern class in the general population, accounting for 60%–65% of all human prints.

What is a Loop?

100

In modern and legacy DNA labs, this step is performed first to break open biological cells and isolate pure DNA strands.

What is Extraction?

100

This chemical process is used in modern forensic labs to make millions of copies of target STR loci from small evidence samples.

What is PCR (Polymerase Chain Reaction)?

100

When the Yekaterinburg mass grave was excavated in 1991, anthropologists determined only 9 bodies were present, meaning this many execution victims were missing.

What is 2 (Prince Alexei and Princess Anastasia/Maria)?

100

In federal database standards like CODIS, this exact number of core STR loci is currently examined to establish an official match in court.

What is 20 Core Loci?

200

If an imaginary line drawn between two deltas touches or crosses central core ridges, the whorl is classified as this specific type.

What is a Plain Whorl?

200

These biological "molecular scissors" are used during RFLP profiling to cut extracted DNA strands into smaller fragments at specific base sequences.

What are Restriction Enzymes?

200

Forensic DNA fragments move toward the positive electrode (+) during gel electrophoresis because of the net negative charge carried by this chemical group.

What is the Sugar-Phosphate Backbone?

200

Forensic anthropologists determine whether a skeleton belongs to an individual over or under 22 years old by checking for the degree of eruption in these teeth.

What are Wisdom Teeth?

200

Rather than sealed plastic Ziploc sandwich bags that trap moisture and destroy DNA, wet biological evidence must always be packaged in this type of container.

What is a Breathable Paper Bag (or Box)?

300

This is the formal term for the study of the uniqueness of friction ridge structures and minutiae points for personal identification.

What is Ridgeology?

300

In legacy RFLP profiling, this X-ray film output displays radioactive probes as a visual "barcode" band pattern for suspect comparison.

What is an Autoradiogram?

300

During gel electrophoresis, these types of STR fragments experience less friction weaving through agarose pores, moving the fastest and furthest down the gel.

What are Shorter STR fragments (fewer repeat units)?

300

All five Romanov children inherited 100% of their mitochondrial DNA (mtDNA) strictly from this royal parent.

Who is Tsarina Alexandra?

300

Damp or wet biological crime scene evidence should never be stored in airtight plastic Ziploc bags because trapped moisture causes this issue.

What is bacterial/fungal mold growth that degrades DNA strands?

400

To classify whorl patterns, an examiner draws an imaginary line between these two triangular ridge structural points.

What are Deltas?

400

In the 1983–1986 Narborough Murders case, this 17-year-old became the world's first person exonerated by forensic DNA profiling after falsely confessing.

Who is Richard Buckland?

400

At a crime scene, hair evidence yields an STR profile that includes this locus, showing X and Y bands to indicate a male donor.

What is the AMEL (Amelogenin) locus?

400

This living British royal family member provided the maternal reference mtDNA used to confirm the identity of Tsarina Alexandra and her daughters.

Who is Prince Philip (Duke of Edinburgh)?

400

To prevent cross-contamination at a crime scene, separate items like bloody fabric and cigarette butts must always be collected and stored in this manner.

What is separately in individual, labeled containers?

500

Matching a fingerprint pattern class (like a Loop) is only class evidence; an examiner must compare these specific ridge characteristics to establish a 100% individual match.

What are Minutiae Points (or Ridge Characteristics)?

500

This killer attempted to cheat the world's first police DNA dragnet by paying a coworker (Ian Kelly) to take his blood test for him.

Who is Colin Pitchfork?

500

Under this foundational rule of DNA interpretation, if a suspect matches crime scene evidence across 19 core loci but differs at just 1 single locus, they are 100% excluded as the donor.

What is the Rule of Exclusion?

500

Forensic scientists conclusively proved that claimant Anna Anderson was NOT Princess Anastasia, but rather this Polish factory worker, using tissue mtDNA.

Who is Franziska Schanzkowska?

500

Modern forensic laboratories analyze hyper-variable non-coding STR regions for CODIS profiles rather than coding genes for this main reason.

What is to identify individuals uniquely without revealing private personal/medical genetic traits?