Personal Care
This vital sign is often the first taken during patient admission.
What is temperature?
This is the shape of the DNA molecule, consisting of two strands twisted around each other.
What is a double helix?
What is the purpose of PCR?
What is to amplify DNA?
Proteins are made of these monomers.
What are amino acids?
What type of organism is E. coli?
What is a bacterium?
According to the CDC, hands should be scrubbed for at least this many seconds when washing with soap and water.
What is 20 seconds?
These four nitrogenous bases make up DNA.
What are adenine, thymine, cytosine, and guanine?
This enzyme is used in PCR to synthesize new DNA strands.
What is Taq polymerase?
The three-letter abbreviation for the structure formed by α-helices and β-pleated sheets.
What is the secondary structure?
What is an incubator used for in microbiology?
To grow cells at a controlled temperature.
This is the correct order to don PPE.
What is gown, mask, goggles/face shield, gloves?
This technique is used to separate DNA fragments based on size.
These short single-stranded DNA sequences initiate DNA synthesis in PCR.
What are primers?
This type of bond stabilizes the tertiary structure of proteins, especially between cysteine residues.
What is a disulfide bond?
What are antibiotics?
Drugs that kill or inhibit bacteria.
Used linen should be placed in this container immediately after removal.
What is a soiled linen hamper or dirty linen bag?
What is Chargaff’s rule?
The amount of A = T, and C = G in a DNA molecule.
This is the name of the three basic steps in each PCR cycle.
What are denaturation, annealing, and extension?
This method uses gel electrophoresis and antibodies to detect specific proteins.
What is a Western blot?
What is the name of the first immortal human cell line?
What is HeLa?
This is the correct term for the opening of the intestine through the abdominal wall.
What is a stoma?
What is the function of DNA ligase?
What is to join Okazaki fragments on the lagging strand?
This piece of equipment is required to rapidly change temperatures during PCR.
What is a thermal cycler?
Touchdown PCR improves specificity by gradually lowering this parameter in successive cycles.
What is the annealing temperature?
This technique is used to isolate a single bacterial species from a mixed sample using a loop across agar.
What is streak plating?