Safety
Name 3 common laboratory hazards
What are acid & bases, biological agents, compressed gases, corrosives, cryogenics, electrical, flammables, halogens, hydrocarbons, mercury, oxidizing & reducing agents, peroxides, pyophorics,
What is the name of the sampling port on the bioreactor?
What is the clave port?
What two pieces of equipment can you use to transfer liquids?
What is a serological pipet, a micropipet?
How do you prepare a sample to run on an SDS-PAGE gel?
What is add SDS and heat?
What kind of cells did we use to make our medicine?
What is CHO or Chinese Hamster Ovary Cells?
What is this symbol called?
What is the NFPA safety diamond?
How long does it take to achieve homogeneity in the Wave bioreactor?
What is 5 - 10 seconds?
What type of syringe do you use to sample the bioreactor?
What is a luer lok syringe?
What is the name of the stain used to stain the gel?
What is Coomassie Blue?
Name three features of an antibody?
What are heavy chain, light chain, disulfide bond, Fc region, constant region, Fab region, variable region
What can you wear to protect yourself in the lab?
What are safety glasses/goggles, lab coat, gloves, close toed shoes
Name the four analyses or tests that are performed on a bioreactor sample.
What are pH, cell count, cell viability and glucose?
What is the small vessel called that we grow the cells in?
What is a Shake Flask?
What protein standard runs at 97.4 kDa (kilodaltons)?
What is Phosphorylase b?
Calculate what volume of 100% ethanol you need to make 500 ml of 20% ethanol.
What is (100%) x (x ml) = (20%) x (500 ml) solve for x x = 100 ml
What does this symbol mean?
What is Flammable?
Who invented the Wave bioreactor?
Who is Vijay Singh
What equipment do you use to mix a solution?
What is a stir plate or stir bar?
What tool do you use to separate the gel frame?
What is a gel knife?
What is meant by the "upstream" and "downstream" parts of the medicine making process?
What is Upstream = the cell culture portion of making the medicine Downstream = the purification portion of making a medicine
What do three of the four regions of this symbol, signify?
What are Blue = Health Hazard Red = Fire Hazard Yellow = Reactivity White = Special Hazard
Name four of the five parameters for the bioreactor that are recorded on the log sheet
What are rocks per minute, angle, percent % CO2, temperature, aeration?
What two methods can you use to separate the cells from the medicine?
What is a filter or filtration, centrifuge or centrifugation?
What does SDS-PAGE stand for?
What is Sodium Dodecyl Sulfate - Poly Acrylamide Electrophoresis?
Name all seven steps in purification or chromatography, in order.
What is Equilibration, Load, Wash, Elute, Neutralize, Sanitize, Store