Hardware
The default gas for FC200
Oxygen
Name all the components included on the Cellicon tubing assembly.
Aseptiquik connectors, Levitronix feed pump, 3X pressure sensors, SU permeate flowmeter.
EM stands for...
Equipment Module
To reduce or eliminate oscillations of the present value about the setpoint, I would adjust this parameter.
I value - Reset
The highest cell density that has been achieved in the iFlex system, so far.
126e6 cells/mL
The preconfigured function for Pump P007 on the perfusion tower.
Cell Bleed
The number of small addition lines provided on top of the bag.
5
List the control modes, in order of increasing automation.
MAN/ROUT, AUTO, CAS/RCAS
The minimum working volumes of the 200L and 2000L bioreactors.
40L and 400L.
The approximate kLa of each sparger.
Open pipe = 5
Mid-range = 50
High performance = 100
The number of E-Stops available on the system
3
This sparger is located on the left-hand side of the 200L bag, when placed into the tank.
Mid-range / drilled hole
For certain PID control loops, this mode indicates that the PID algorithm is not used by the module. Instead, the setpoint is used to immediately determine an output.
bypass mode
TMP, Pressure Drop, Flux, Throughput
All bioreactors achieve less than this mixing time.
34 seconds
The MFC output options for the optional FC500, FC600, and FC700 on the 200L system.
5, 10, 20 or 50 SLPM
Two potential issues during the bag installation process.
Many will be accepted...
Name 3 purposes for the engineering laptop.
User management, recipe development, troubleshooting
Name 7 of the available calibration workflows.
pH, DO, CO2, VCD, Flow sensor zero, pressure sensor zero, pump flow control, pump speed control, tare weight
The location of the highest shear zones within a stirred tank bioreactor.
The impeller zone
The accuracy of the DO sensor, pH sensor, and Temperature probe
DO +/- 10% of MV; pH +/- 0.1; Temp +/-0.2 degC
Name each of the available ports on the side panels of the bag.
Right: VCD slot, 3x MU probe slots, cell bleed, CRD return, 2X sampling lines
Explain the difference between a phase, operation, and unit procedure.
A Unit Procedure defines the strategy for carrying out a contiguous process within a unit. (Bioreactor Perfusion Process) An Operation contains one independent processing activity. (Liquid Addition) A Phase is the specific occurrence of an operation. (Antifoam Addition)
List the steps of executing an EM for DO Operation.
1.Select SP, Flex Profile, Device Assignments, and Gas Destinations from Operating Parameters. 2.Run the Enable Command from DO EM. 3. Verify DO EM Command Completes
Two sources of deviation in the control performance, experienced during internal cell culture runs.
Antifoam addition - to DO in fed-batch run; Feed additions using cold media - to temp in fed-batch run; PLC issue - perfusion run