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Syn Bio
iGEM
All things DNA
Know your friends
Misc.
100
This circular piece of DNA is naturally exchanged between bacteria and is used by synthetic biologists to engineer functions in bacteria.
What is a plasmid
100
The name of the 2016 Harvard iGEM team's project this year.
What is Plastiback
100
The most precise method of editing DNA, this method was popularized by its use to edit genomes in human cell cultures in the Church and Zhang labs.
What is CRISPR/Cas9
100
These compers helped organize and accompany a trip to Gingko Bioworks.
Who is Cathy and Hyeon-Jae
100
We are going to watch this movie at the comp celebration during reading period.
What is Gattaca.
200
Using this CRISPR-based system, invented by Kevin Esvelt and George Church at the Wyss institute, a genetically modified organism can be guaranteed to pass a trait to all of its progeny, spreading through a population.
What is a gene drive?
200
iGEM began in 2004 with five teams from different schools and was hosted by this school.
What is MIT
200
Used in many lab protocols, this reaction amplifies a DNA fragment. Some necessary components of this reaction include a DNA polymerase and DNA pieces known as primers.
What is PCR (Polymerase Chain Reaction).
200
This comper is in the class of 2019.
Who is Santiago
200
This Harvard Square business specializes in making bubble tea, served hot or cold in a sealed cup.
What is Boston Tea Shop.
300
Although synthetic biology usually engineers the bacteria E Coli, other types of organisms are used, including mammalian cells and plants. This type of plant was engineered to make a precursor to vitamin A and was promoted as a solution for areas with vitamin A deficiency. However, it was met with opposition from anti-GMO organizations.
What is rice / Golden Rice.
300
Earlier this year we played a game where you guessed what was a real and what was a fake iGEM project. This project was real and based off a popular computer game called what? (200 pts. for name of project, 100 pts. for game name)
What is Colisweeper and Minesweeper
300
This molecular biology procedure is used to construct plasmids by joining overlapping DNA fragments.
What is Gibson Assembly
300
This board member is in charge of iGEM club's finances.
Who is Daniel Shen.
300
This is the room where iGEM comp meetings take place.
What is Boylston 104.
400
One of the most well known achievements of synthetic biology was engineered yeast that produced a precursor to the molecule artemisinin, a treatment for this disease.
What is malaria
400
The goal of an iGEM team is primarily to produce these units of DNA, which are then put into the Registry of Standard Biological Parts.
What are Biobricks
400
When designing a DNA construct to express a gene in bacteria, add this sequence upstream of the coding sequence to recruit this protein-translating enzyme. (200 pts for each part)
Ribosome binding site (RBS), ribosome
400
The 2015 iGEM team (Ethan's team) worked on this project, which sought to make bacteria stick to a target (such as cancer cells).
What is Bactogrip.
400
This is the operating system of Ethan's computer.
What is Linux
500
This is a system of three regulatory genes, in which each gene represses the next. The result is alternating expression of a protein, such as the fluorescent protein GFP.
What is the repressilator
500
The grand prize winning team of 2016.
What is Imperial College. They weren't that great.
500
For both DNA and RNA, these fragments stick to something in particular. The Heidelberg 2015 team wrote a software to generated them and another popular method is called SELEX.
What is an aptamer?
500
This is Rena's favorite snack from CVS.
What are maple cookies.
500
This quote, by a famous physicist, was encoded in the first cell with a completely synthetic genome by Craig Venter, along with web address.
What is "What I cannot create, I do not understand"?