Cleave Away
Name something that ficin enhances that we wish it wouldn't.
What is an autoantibody? In particular, a cold autoantibody such as Auto-anti-I.
These documents provides information about the hazards, safe handling and emergency measures of a chemical or substance.
What is a Material Safety Data Sheet (MSDS) or now more commonly called a Safety Data Sheet (SDS)?
This is the maximum amount of time you can EGA treat without running the risk of over incubation and doing irreversible damage to the RBCs.
What is 2 minutes?
This is the minimum number of days from transfusion it should be before attempting a microhematocrit cell separation.
What is 3 days?
Name one system of antigens are destroyed by EGA that is also destroyed by DTT.
What is Kell?
All Kell and Lutheran System Antigens as well as CD38 are destroyed by this.
What is 0.2 M DTT?
This form must be completed anytime a reagent will be prepared and stored for later use.
What is 16.4.frm087?
This is the acid used in the EGA test to remove the anti-IgG from the patient cells.
What is Glycine Acid?
What is 2-5 mm?
This can be used to treat cells prior to adsorption thereby enhancing the adsorption by freeing up antigen-antibody binding sites on the red blood cells.
What is Ficin (or Papain)?
Ficin and Papain are examples of these types of enzymes that cleaves some of the glycoprotein chains off of red cell membranes.
What are proteolytic enzymes?
What is the NFPA (National Fire Protection Association) diamond symbol?
Treatment with this reagent/chemical for removal of anti-IgG has a maximum treatment time of 2 hours and the DAT is tested every 30 minutes.
What is CDP or Chloroquine Diphosphate?
This is used to seal the microhematocrit capillary tubes prior to centrifuging in performing a cell separation.
What is Clay?
This is the final step of preparing a reagent and documenting all steps on 16.4.frm087.
What is obtaining a second person review?
Name at least 4 antigens that is removed/destroyed by Ficin or Papain.
What is Fya, Fyb, M and N? (Also S and s can be destroyed but are variable).
This Reference procedure gives instructions need for Preparing, Labeling and Storing Reagents prepared in-house that will be used for later use.
What is 16.4.ref054?
This reagent which is part of the EGA kit should be kept at Room Temp to prevent crystallization.
What is EDTA?
This is how the microhematocrit cell separation works.
What is by density?
This enzyme can be used to remove CD38 but does not destroy the antigens of the Kell system.
What is Trypsin?
Name a test that should always be tested when ficin treating cells for serological or antibody identification.
What is an autocontrol?
OSHA requires this type of label be applied when preparing reagents that are defined as hazardous.
What is a Hazcom label (or the NFPA label)?
Name a control that must be tested in parallel with antigen typing when using EGA or CDP treated cells.
What is a 6% albumin control?
This has to be occurring within the patient's body in order for the microhematocrit cell separation to work.
What is the patient has to be producing reticulocytes?
This gives a 3-4+ reaction at Immediate Spin which proves a cell has been treated with Ficin or Papain.
What is Glycine Soya?