Jeopardy

Acronyms

100

SCC

Safe Clean Compliant

200

UFDF

Ultrafiltration/Diafiltration

300

HETP

Height Equivalent to a Theoretical Plate

400

IQOQ

Installation Qualification Operational Qualification

500

"VPE" in "SoloVPE"

Variable Pathlength Extension

Upstream Without A Paddle

100

This is added to reduce foaming in a bioreactor

Antifoam

200

This is utilized when the pH of a bioreactor is too high

Carbon dioxide

300

Which of our bioreactors in PA2S are considered expansion reactors

100L and 500L

400

The process of moving the cell culture to a larger bioreactor

Inoculation

500

Antifoam addition timepoints are based what?

Inoculation end time

Who's Who?

100

I am a Lead Cleaning Technician

Esko Mason

Garold Rabinovich

Wade Rathbun

Henry Heroux

Michael Mateo

Dennis Brown

Dan Gegenheimer

200

I am the President and CEO of Regeneron

Leonard Schleifer

300

I am in the Lead or Supervisor role for the Process Techs

Liberato Parillo (Lee)

Phillip Morelli

Jonathan George

William Johnson

Scott Maccue

John Fountain

Daniel Kern

Stephan Viger

Nicholas Rockstroh

Bryan Riegel

Jamie Keys

400

I am in the Upstream Supervisor role in PA2S

Alex Betrus

Ben Callahan

Greg Johnson

500

I am a support tech for SUM

Felicia Pease

Clive Thom

Kiley Brown

Kimberly Teal

Nicholas Butler

Mariano Colon

Logan Pugsley

Iris Maisonet

Joshua Metzger

Can I Be Skid Princess?

100

AS152 would indicate air is where in the skid?

Prior to the column

200

PIR111 would indicate there is pressure where on the skid?

Prior to the bubble trap/filter

300

What visual indicator shows that solution will BYPASS the column hoses/column

Diagram on the lower right of the HMI will be highlighted green going around the column

Text Instruction states BYPASS

400

Looking at this on the skid HMI could tell me if I am utilizing the incorrect NaOH solution

Conductivity

500

Name the solutions in the order in which they are used when performing HETP

0.1M NaCl

1.0M NaCl

0.1M NaCl

Cast Your Worries Downstream

100

This operation is utilized to denature any theoretical viruses in the product

Viral Inactivation

200

Operation used to remove product from the UFDF skid following Final Concentration

Product Recovery

300

This process removes impurities from the product based on the charge

Anion Exchange Chromatography or Cation Exchange Chromatography

400

This type of buffer would coat the protein to help reduce aggregation when freezing

Excipient Buffer

500

Product is unbound from the resin bed and collected during this ProA block

Elution