Biology
This sugar induces expression rather than lactose in the pET system
What is IPTG?
The NMR solvent peak at 7.26 ppm
What is CDCl3
This cartoon cat hates Mondays and loves lasagna
Who is Garfield?
Protein engineering by iterative rounds of mutation and selection, run in the lab on a timescale of weeks
What is directed evolution?
What are the STOP codon sequences?
Merck and Codexis replaced a rhodium hydrogenation with an engineered transaminase in the synthesis of this drug
What is sitagliptin?
This person just got a cat like a few weeks ago
Who is Esther?
Used to inoculate plates, distribute buffer, transfer samples to Axygen/Waters, and it prob gives you carpal tunnel
What is a multichannel pipette?
This technique, conceived by Kary Mullis on a California highway drive, earned him a Nobel eight years later
What is PCR?
Unlike kinetic resolution, which caps at 50% yield, DKR can theoretically reach this
What is 100%?
Shrek's sword-wielding sidekick
Puss in Boots
What is the rapidfire (RF)?
Whether a protein binds an anion exchange column at a given pH is set by this parameter
What is the pI (isoelectric point)?
The Buchwald-Hartwig amination forms a C-N bond, and its rate-determining step in most catalytic cycles is this
What is reductive elimination?
Cats can't taste this flavor
What is sweet?
Do this when yields are high (enough) and you don't want to make authentic standards
What is scaling up?
This degenerate codon is preferred over NNN because it encodes all 20 amino acids in just 22 combinations
What is NNK?
This pericyclic reaction of a vinyl allyl ether is [3,3] like the Cope, but the oxygen in the chain makes it irreversible by delivering a carbonyl
What is the Claisen rearrangement?
Archer and Bingley
What are the names of Maddie's (and Caroline's) cats?
Loves charli xcx, it's her last day today, matcha queen, gay <3
Who is Katie Jane Torma?