DNA cloning
Molecular techniques
DNA cloning
100
Complementary copies of mRNA
What is cDNA
100
Analytical technique used to detect specific proteins in the given sample.
What is a western blot
100
Short strand of nucleic acids.
What is an oligonucleotide
200
Recombinant DNA molecule containing a DNA sequence or a gene.
What is a clone
200
Genetic variation that can be detected by restriction enzyme digestion
What is a RFLP.
200
Collection of clones, each of which carries vector molecules into which a different fragment of DNA has been inserted. It should theoretically contain all of the sequences found in the original DNA source.
What is a library
300
DNA molecule that can replicate autonomously in a host cell
What is a vector
300
RNA blotting Standard approach for determining the size and the abundance of the mRNA from a specific gene in a sample of RNA.
What is a Northern blot
300
Enzyme that pairs the complementary overhangs followed by rejoining the phosphodiester backbones on each strand.
What is a DNA ligase
400
Cloned DNA or RNA molecule labeled with radioactivity used to identify its complementary sequences by molecular hybridization
What is a probe
400
technique that allows to find or examine DNA that was previously cleaved by restriction enzymes.
What is a southern blot
400
Ligation of DNA molecules from different sources (like a fragment of DNA and a vector).
What is recombinant DNA technology or molecule
500
Fragment of DNA cloned into a vector.
What is an insert
500
dNTPs, master mix, MgCl2, DNA, Taq pol, water, primers
What is a PCR mix?
500
DNA enzyme that uses mRNA as a template.
What is reverse transcriptase