Conduct and Situational Awareness/PPE and Attire
Which of the following is never tolerated in the laboratory? Horseplay, Following all instructions, Telling instructor about incidents or pointing test tube away from individuals?
Horseplay is never allowed. DONT EVEN THINK ABOUT IT.
what is I will keep my work orderly and safely stored? what is I will decontaminate my work area, return unused equipment and wash my hands thoroughly and what is I will wait to be dismissed by the instructor once cleanup has been verified?
Fill in the blank I will treat all _________, cultures, specimens and contaminated work surfaces as potentially hazardous even if they are nonpathogenic or simulated.
What is biologcial materials?
Thomas grabbed the first microscope and went to his desk and began working. What lab rule did he miss?
What is I will inspect equipment before use for damage and report it immediately?
Michelle notices there are alot of boxes strewn about the classroom blocking both exits and the chemical shower and eyewash station. What safety rule was broken here and how can it be remedied? Please answer to both parts.
Safety rule being broken: What is do not block exits or pathways to emergency systems. Boxes need to be stored in proper areas.
What reagent do we use when decontaminating work surfaces, especially when working with bacterial cultures? Hint: It was an SDS we had from previously
What is Ethanol?
How many sections are present on the SDS sheet?
Marisol cleaned up the laboratory after use. Put all her materials away and then promptly left the lab. What did she forget to do that relates with sterile technique before you leave the lab every time?
What is she forgot to wash her hands?
When micropipette do you select the micropipette with the smallest volume size that covers your determined volume or can you use any micropipette that will draw up the amount specified?
What is you use the smallest micropipette that will draw up your intended volume? Using a larger pipette introduces inaccuracy and uncertainty in your measurements as larger volume pipettes rather than smallest standard range have the ability to capture more air.
I am responsible for knowing where nine following items are located? Name all nine of them (hint all nine are your notebook when we made the map)
What are the?
Fire extinguisher
Exit
Eye wash
Fire blanket
Storage room
Your current desk
Teacher desk
Sinks
Trash can
In our forensics, What is absolutely prohibited? Name any potential infraction associated with the rules.
Hint: Check your lab safety contract on this one and this one only.
What is any of the following?
I will not bring personal samples to class
I will not use lab to test personal activities unless instructed to do so.
I will remain academically honest.
Analyze the three following pictograms and tell me what they stand for from the SDS sheet for Ethanol.
Pictogram one: Red Diamond with fire inside it
Pictogram two: Red Diamond with exclamation Mark inside it
Check the SDS I am going to give you.
What is
Flammable
Can Cause irritation
When culturing stock bacterial colonies, all handling of bacteria must be done in what location in the laboratory?
What is under the biohazard hood unless the instructor determines otherwise?
How does one balance a centrifuge? Take a guess.
What is you make sure all sides of the centrifuge have test tubes with equal volume such that every side has the same amount of weight?
Nancy came to class prepared or so she thought. She wore googles, a lab coat, and closed two shoes but she is missing one item. Nancy is known for her long black hair. What item did she forget?
What is a hairtye?
Emergency Response: What is the FIRST thing you should do when a possible emergency may be imminent?
What is notify the instructor immediately before anything and all else?
Sally needed to free up her hands to do something else. She was pippetting by mouth, smelling substances directly and even decided to taste a brown liquid cause she was curious. What rules did she break here? Mention all 3
She nasty.
What is no smelling substances directly? What is tasting substance from the lab?
Do gloves replace handwashing?
What is no they do not?
Which of the following rules applies to this situation?
Samuel was wearing gloves, goggles, closed toed shoes and clothing that adequately covered his body. As well as he was chewing gum. What rule did he follow correctly and What rules were broken? Answer is two parts.
What is wearing the proper PPE every time it is assigned on lab day and what is no food or drink shall be permitted in the laboratory at any time?
WHat is return all materials to proper places and never pour chemicals down the sink unless approved by instructor?
Could we dispose of the following solution down the drain?
A concentrated solution of Sodium Chloride.
Yes, Sodium Chloride is simply salt water. In this instance, you can pour down the drain safely as long as it is not mixed with any other reagents.
These microbiological safety rules were derived from what government agency responsible for determining the guidelines involved in safe procedures when working with biological materials?
What is the CDC or the NIH?
Where do you dispose of broken glass? How should it be handled? Tell me the steps as if you broke a beaker
What is
NOTIFY INSTRUCTOR
Get broom and dust pan and sweep pieces into it
Lastly, dispose in the broken glass container.