Sequencing: The Next Generation
Networks
RNA
Normalization
Clustering
100
This sequencing technique utilizes cyclic reversible terminators.
What is Illumina?
100
In a feed forward loop, the final target gene can be controlled by this type of logic.
What is and logic?
100
This is the name for all possible transcripts encoded in a genome.
What is a transcriptome?
100
This is the type of distribution that best characterizes RNAseq data.
What is negative binomial?
100
This name sounds like you have no clue but instead describes clustering methods used to identify dominant patterns without any prior knowledge.
What is unsupervised?
200
These two methods utilize a different fluorophore for each nucleotide type.
What is Illumina and Pacific Biosciences?
200
The three most common network motifs in biology.
What are FFL, SIM and DOR?
200
This is a type of RNA that can be sequenced easily by use of an oligo dT primer.
What is an mRNA?
200
The two areas of the box plot graph that split and the lowest and highest 25 percent of the data is examined.
What are the whiskers?
200
This distance metric looks at the shape of expression profiles across a number of conditions.
What is Pearson correlation?
300
This molecule is released when polymerase adds a nucleotide to the growing strand.
What is pyrophosphate?
300
A gene type that can be both an input and an output in a gene regulation network.
What is a transcription factor?
300
This RNA is smaller than most and contains the name of an 80’s eccentric child entertainer.
What is a piwiRNA?
300
For microarrays, this is the best method to identify differentially expressed genes between multiple samples.
What is an ANOVA?
300
Genes within clusters likely contain the same types of these within their promoters?
What is a cis-element?
400
This is the name of the nanophotonic structure used in Pacific biosciences which enables single fluorophore detection.
What is Zero Mode Wave Guide?
400
) A network motif common in metabolism, because it allows the sequential activation of a group of genes by a single input signal.
What is a SIM?
400
This type of RNA is very abundant and needs to be removed when carrying out RNAseq analysis.
What is rRNA?
400
This method of normalization looks at the number of fragments and transcript length.
What is FPKM?
400
When trying to figure out what genes in a cluster do, you can use this type of classifier that is laid out in a directed acyclic graph.
What is a Gene Ontology term?
500
This was done to the DNA polymerase in Pacific Biosciences to allow nucleotides with phoshpholinked nucleotides to retain near wild type polymerase kinetics.
What is mutation?
500
In the E.coli gene regulatory network used to identify network motifs, motifs of this number of nodes were considered.
What is three?
500
This type of RNA was found to regulate cell proliferation in prostate cancer.
What is long intergenic non-coding RNA?
500
This is the type of transformation that tends to be applied to data to look at relative expression.
What is log transformation?
500
Size matters. OR so says this particular type of distance metric.
What is Euclidean distance?