Article Specifics:
What three stressors is HIF-1 known to mediate resistance in C. elegans?
1.) Immune: pathogenic bacteria Pseudomonas aeruginosa
2.) ER stress: tunicamycin-induced ER stress
3.) Oxidative Stress: oxidative stress from tert-butyl hydroperoxide
What is RNA-seq?
RNA-seq is a method adapted to measure the gene expression in cells through mRNA transcript production. This allows us to see which genes are active and how much they are transcribed thereby telling us which genes are active in mutated cells.
What mutant is most likely to result in the locomotion speed highlighted?
egl-9(lf)
What was the name of the specific allele in the cyp-36A1 gene that contained a missense mutation?
n5666
What is the goal behind screening for second site mutations?
In many cases, the second-site mutation suppresses the first mutation, so that the double mutant has the wild-type phenotype.
What gene is most likely represented by the diagram?
cyp-36A1
How many genes are regulated by hif-1 in hypoxia-exposed worms?
63
The paper mentions that animals which have mutations in C. elegans VHL homolog vhl-1 are resistant to these stressors relative to wild-type animals. Why may this be the case?
Where could you find this level of expression of PT24B8.5::gfp for a cyp-36A1(+) transgene?
Intestine