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100

How can Bacillus anthracis and Bacillus cereus be told apart?

Bacillus cereus is motile and penicillin resistant

Bacillus anthracis is nonmotile and penicillin susceptible

100

What disease is caused by Coxiella burnetti?

Q fever

100

What organism causes purulent conjunctivitis?

Haemophilus influenza biogroup aegyptius 

100

What is the difference between antiseptic and disinfectant?

Antiseptic is used on body 

Disinfectant is used on nonliving surfaces such as counter

100

What percentage of individuals get reactivated tuberculosis?

5-15%

200

Lymphogranuloma venereum (LGV) is caused by what serotypes of Chlamydia trachomatis?

L1, L2, L2B, L3

200

A neonatal has meningitis. The organism was grown on CAMP with S. aureus and created a rectangle and is BEA positive. What organism is suspected?

Listeria monocytogenes 

200

This organism is a gram positive spore forming bacilli and produce Toxin A & B. This organism grows on Cycloserin cefoxitin fructose (CCF) and C. difficile selective agar (CDSA). 

Clostridium difficile 

200

Urine specimens are cultured using what and what method?

Calibrated loop

Semiqualitative way (line down the middle then wave)

200

This organism presents with a metallic green sheen on EMB, IMVC ++--, and ONPG positive  

Escherichia coli 

300

The difference between the acid fast stains are? What are they used for?

Auromin-rhodamine is screening for mycobacterium, Ziehl-Neelsen and Kinyoun is confirmation.

Auromin-rhodamine is a fluorochrome stain (fluorescent). Ziehl-Neelsen is a heat. Kinyoun is the cold. 

300

A patient with chronic granulomatous disease grew an organism on SBA that was yellow, serrated, smooth colony. This organism is?

Burkholderia cepacia 

300

This organism was gram positive in clusters. The clusters were confirmed by a positive catalase. The analyst performed a tube coagulase test and it was negative. How would you differentiate between Staphylococcus epidermis and S. saprophyticus?

Novobiocin

S. saprophyticus has inherited novobiocin resistant

S. epidermis is susceptible to novobiocin. 

300
What is the key characteristic of Chromobacterium violaceum?

Violet pigment on SBA

300

This organism is a slow grower that is non-spore forming, partially acid fast, branching gram positive that grows on BCYE.

Nocardia 

400

Lowenstein Jensen (LJ) selects for who? and is what based media?

Mycobacteria

Egg based

400

This organism will have no carbohydrate reactions.

Moraxella catarrhalis

400

E. coli O157: H7 can progress to what?

Hemolytic Uremic Syndrome

400

What is the confirmatory test for syphilis?

Treponemal tests for antibody

FTA-Ab, TP-PA, EIA 

400

What is the leading cause of gastroenteritis in the U.S.?

Campylobacterium jejuni

500

Shigella sonnei (D serotype) will have what results for Mannitol fermented, ODC, ONPG?

Positive 
500

This organism is a bipolar staining resembling a safety pin. The organism optimum growth is 25-32C. The organism is nonmotile at 25C. 

What disease does it cause?

Yersinia pestis

Black plaque 

500

This organism has botulism toxin that is found in home canned foods and why you can't give honey to babies under 2 years old. 

Clostridium botulinum

500

What are the stages of Treponema pallidum subsp. pallidum? What are the key characteristic factors?

First- painless chancre

Second- rashes on palm of hands and sole of feet

Latent- absence of disease and positive serology

Teritary- CNS involvement 

500

This organism is gram positive chains, beta hemolytic and had mucoid colonies, and was bacitracin susceptible. What organism is suspected?

Group A Streptococcus (Streptococcus pyogenes)