Acronyms
What does SC mean?
Superior colliculus
How were the animals in this experiment genotyped?
PCR
Which was your favourite figure?
1-8
Does EphrinB have a higher concentration in medial lateral of SC?
Medial
Who presented the background information?
Emilyyyyyyyy
What does TZ refer to?
Terminal Zone
Which type of animal(s) did the experiment use?
EphB mutant mice
How many figures were presented in this journal article?
8
What type of mapping did this study primarily look at?
Dorsal-ventral
Who did not present a figure in this journal club?
Emilyyyyyy
What is the D-V axis? L-M?
dorsal-ventral retinal axis, lateral-medial axis.
Name the three methods used.
In Situ Hybridization, Immunohistochemistry and Anterograde Axon Labeling and Quantitation
What did figure 1 generally show?
hint: what expressed? And in where?
EphB and Ephrin-B Expression in the Retina and Superior Colliculus
Where did the ectopic terminal zones develop in EphB2 and EphB3 mutant mice?
Lateral to the normal TZ.
What is the title of the journal article?
EphB Forward Signaling Controls Directional Branch Extension and Arborization Required for Dorsal-Ventral Retinotopic Mapping
What is T-N, A-P, and RGC?
Anterior-posterior, temporal-nasal, retinal ganglion cells.
Name five of the eight probes used in In Situ Hybridization.
EphB2, EphB3, EphB4, EphB6, ephrin-B1, ephrin-B2, ephrin-B3, and Vax2.
Figure 3 showed a Retinocollicular Map Development in what?
Wild type mice
Where are EphB's expressed?
In the ventral retina
What is the goal of this study?
To understand how EphB and EphrinBs interact and contribute to branch extension and arborization in D-V retinotopic mapping,
What is EphB3 short for?
Ephrin type-B receptor 3
What complex reacted with the DAB substrate in the immunohistochemistry method?
avidin-biotin-peroxidase
In figure 6, researchers used transgenic mice to demonstrate that ______ _______, which is mediated by EphB2 and EphB3 expression, is required for mapping the D-V axis of the retina along the L-M axis of the SC.
forward signalling
EphB2 and EphB3 have decreased ability to extend _________.
hint: medially, dorsally, ventrally, laterally, or nasally.
Medially
Name one future directions for this journal article that McKenna discussed.
1. Mapping defects in mechanisms for nasal RGCs
2. Other receptors involved in D-V mapping → EphB4, EphB1?
3. Analyzing targets of genes (i.e. Vax2, Tbx5,) that regulate axial polarity in the retina