Extraction
DNA is contained within this cellular structure
What is the Nucleus/Nuclear Envelope?
NMDPS uses this instrument to quantify DNA after extraction
What is the ABI 7500 Real Time-PCR System?
Kary Mullis conceptualized this technique for amplifying the amount of workable DNA in a sample
What is PCR?
The 3500 genetic analyzer uses this number of capillaries
What is 8?
For NMDPS forensic laboratory, this is the minimum number of loci required for profile interpretation
What is 10?
This term is used to describe a chemical that specializes in cutting open cells
What is a Lysing Agent?
The standard curve plots these two values against each other on the X and Y axese
What are Cycle Threshold (Y-axis) and Log[DNA Concentration] (X-axis)?
These are the focus of DNA amplification
What are STR's?
The size standard used by NMDPS forensic laboratory is called
What is GS600 LIZ?
The analytical threshold for autosomal STR evaluation is
What is 100 rfu?
Chelex, a commonly used extraction method, binds to metals like manganese, magnesium, iron and other metals of this valency
What is Divalence or Divalent Ions?
Quantifiler Trio utilizes these four target assays
What are: Small Autosomal, Large Autosomal, Male, IPC targets?
The four primary steps in PCR are
What are Hot Start, Denaturation, Annealing, Extension?
A DNA fragment's mobility through the capillary is determined by these two factors
What are Charge and Size?
We use different color channels to differentiate these
What are STR's of Similar Size
The DNA IQ/Maxwell system utilizes this phenomenon, studied by Michael Faraday in the 19th century, to selectively accumulate resin beads during supernatant removal steps
What is Paramagnetism?
Our forensic laboratory uses this range of DNA concentrations to build a standard curve
What are 50 ng - 0.005 ng?
NMDPS uses the Globalfiler kit to amplify DNA because it includes this all of these
What are CODIS Required Loci?
What is Polymer Concentration?
These two loci are the most discriminatory due to their size
What are SE33 and FGA?
This extraction method, developed by Lee Burgoyne at Flinders University in Australia, utilizes a cellulose based material and multiple chemicals to extract and stabilize DNA from blood
What is FTA Paper?
Quantification master mix must have these two ingredients to function
What are dNTPs and Taq Polymerase?
Globalfiler uses these five dyes to create color channels during CE
What are: 6-FAM, NED, TAZ, VIC and SID?
This scientific phenomenon is responsible for the movement of DNA through the capillaries
What is Electro-osmotic flow
Generally, stutter is more commonly observed in this type of nucleotide repeat sequence
What is Dinucleotide Repeat?