Carboys
Autoclave
Cleaning
Cleaning/Tubing
Wrapping
100

How many tallies should be on a carboy before soaking them?

What is 10

100

How often are the status signs updated?

What is before each run

100

How often are brushes cleaned?

What is daily.

100

How long is the WFI water good for after opening?

What is 7 days.

100

What is the purpose of blue paper and breather bags?

What is to protect items.

200

How many tallies are on a carboy in order for it to be tossed?

What is 25

200

Why should load patterns be followed?

What is validation

200

What items need to be cleaned with CIP?

What is transfer metals, metal elbows, and metal barbs.

200

How often is the Liquinox changed?

What is every morning and when there is debris.

200

Why are lids wrapped separatley?

What is steam will not enter the container.

300

What items require tallies?

What is carboys, carboy caps, and tube caps

300

What temperature does the autoclave sterilize the items?

What is 121 degrees C

300

Why are dishes separated between the labs?

What is biofilm

300

Why is it important to replace the tubing?

What is biofilm and cracks

300

Where should autoclave tape be placed on breather bags?

What is on the plastic side

400

What happens to carboys when contamination occurs?

What is throw them away and log the numbers on the carboy log.

400

What PPE is required to load and unload the autoclave?

What is face shield, heat resistant gloves and apron.

400

What frequency is the viafuge UV'd?

What is daily

400

What could happen if a 15 inch was made but should have been 10 inches?

What is contamination

400

Why are items wrapped the way they are?

What is so they are easy to use in the lab.

500

Why do carboys need to be tossed once contamination occurs?

What is potential biofilm on the carboy

500

What cycle is utensils?

What is P1

500

How often is the DI hose changed?

What is daily.

500

Why should tubing lengths be followed and not changed?

What is they were validated.

500

What does the blue paper and breather bags do in the autoclave when sterilizing?

What is the pores open up.