O2 Req
growth throughout tube, concentrated growth at top
What medium is used and what is it made of
Nitrate broth -- nitrate
what medium is used and what is it made of?
Phenylalanine agar -- phenlyalanine
what is a positive result? what is a negative result?
positive - red
negative - yellow
what is a positive result?
+ blue
growth of aerobe and microaerophile
Aerobe: concentrated growth at top of tube
microaerophile: concentrated growth just beneath surface of tube
what enzyme is produced?
nitrate reductase
what enzyme is produced?
phenylalanine deaminase
MRVP broth
basic broth ingredients + dextrose or glucose
what medium is used? what is it made of?
Simmons citrate agar
citrate, bromothymol blue
growth of obligate anearobe and aerotolerant
obligate anaerobe: concentrated growth at bottom of tube
aerotolerant: even growth throughout tube
What reagent is used and what is it made of?
SpotTest reagent C -- zinc
what are the products?
ammonia & phenylpyruvic acid
what is this test used for? what does that mean for enzymes used/products?
detect which fermentation pathway is used
a variety of enzymes are formed, and various organic acids are produced.
what (2) enzymes are produced?
citrate permease and citrase
media used
thioglycollate broth
what is the product and what is produced in medium?
Nitrite ions
gases (CO2 and N2)
What reagent is used and what ingredient is it made of?
Acidic Ferric Chloride. made of Acidic Ferric Chloride.
what is produced in the medium?
acidic-form methyl red
what is a negative result?
green
ingredients of media
sodium thioglycollate
what are the two positive results and one negative result?
2. media is yellow with zinc
1. media is red with zinc
what is a positive result?
what is a negative result?
positive - green
negative - yellow
what reagent is used and what is it made of?
methyl red indicator
methyl red + hydrochloric acid
what products are formed?
Pyruvic acid, oxaloacetic acid, CO2, and Na2CO3