Bioproduction Basics
This nutrient-rich liquid is commonly used to culture cells and microorganisms.
What is growth media?
The Gram stain differentiates bacteria based on differences in this structure.
What is the cell wall?
This piece of equipment spins samples at high speeds to separate components.
What is a centrifuge?
ATCC stands for this.
What is American Type Culture Collection?
A foggy or discolored media flask likely indicates this issue.
What is contamination?
This basic ingredient is often used in bioreactors to provide carbon for cell metabolism.
What is glucose?
This microorganism is often used in baking and alcohol fermentation.
What is yeast (Saccharomyces cerevisiae)?
This item keeps cultures at a consistent warm temperature, such as 37C.
What is an incubator?
The year ATCC was founded.
What is 1925?
Forgetting to tighten this might lead to spilled cultures in a centrifuge.
What is the rotor lid?
This term describes cell lines that grow while attached to a surface.
What are adherent cells?
These bacteria can survive in extreme conditions like hot springs or deep-sea vents.
What are extremophiles?
Used to amplify DNA through thermal cycling.
What is a PCR machine?
ATCC's mission includes preserving and distributing this type of biological resource.
What are reference strains?
Forgetting to include this in PCR can result in no DNA amplification.
What is DNA polymerase (or Taq polymerase)?
In recombinant protein production, this small circular DNA is often introduced into host cells.
What is a plasmid?
This class of virus carries its genetic information as RNA and uses reverse transcriptase.
What are retroviruses?
This tool is used to measure optical density of cultures.
What is spectrophotometer?
ATCC's cell lines are tested for this bacterial contaminant known to affect research.
What is mycoplasma?
Your PCR reaction is producing multiple unexpected bands. What could be the cause?
What is primer-dimer formation or nonspecific annealing?
This bioproduction method uses hollow fibers or cartridges to grow high-density cultures.
What is perfusion culture?
The complete set of proteins expressed by a virus-infected cell is known by this term.
What is the virome (or viral proteome)?
This instrument uses a laser to count and sort cells based on their properties.
What is a flow cytometer?
This collection within ATCC supports the safe handling and identification of BSL2 pathogens.
What is the BEI Resources collection?