The Cell
This cellular structure is responsible for protein synthesis
Ribosome
What is the shape of a DNA molecule
Double helix
What must you always add to your micropipette before taking up solution?
A tip
What is the purpose of a buffer in the chamber
Buffer contains ions and allows for current to run through in order to move molecules (any variation of this response is acceptable)
In the Central Dogma, this is the step where a 3 letter RNA code is used to form proteins.
Translation
The process of copying DNA before a cell divides
DNA replication
The enzyme that generates copies from a DNA template
DNA Polymerase
Which micropipette would you use to measure 125 μL of solution
What is the name of the positive electrode in the chamber
Cathode
This messenger molecule in the cell is transcribed from DNA.
RNA
This is the process used by dividing cells to to create two new identical cells.
Mitosis
An organism that has two identical alleles of a gene is called this.
Homozygous
What is the purpose of a nanodrop?
Quantify and assess the purity of DNA, RNA, and proteins
what is the purpose of a ladder in gel electrophoresis?
To reference and compare bands from PCR products to find the approximate size
Gel green binds to what molecule?
DNA
This is the most abundant molecule in the cell
Water
This genetic element determines where transcription starts
Promoter
What size micropiette is used to measure 1mL of solution?
P1000
what is the purpose of gel electrophoresis?
To study molecules based on size and charge/to make sure you have the desired product from PCR (any variation of this response is acceptable)
In which direction is DNA made (use 3’ and 5’ in your response)
5’ to 3’
This is the part of a human cell that store the genetic material.
Nucleus
This type of mutation would give you varying sized PCR product
Tandem repeats or Insertion or Deletion
What does the A260nm/A280nm ratio tell you when using the nano drop?
DNA quality/contamination
I want to make 10 gels 1.8% gels. How would I go about this? Hint: Include amount of 1X TAE buffer and agarose
2.16g of agarose in 120mL of 1X TAE
what is the complimentary stand of the following sequence
CCATTGCAAG
GGTAACGTTC