Fixation
This is the main goal of fixation
What is to stop autolysis + putrefaction?
The most commonly used method of decalcification is
What is acid decalcification?
Routine processing steps consist of 3. Name us in order.
What is dehydration, clearing and infiltration?
Complete removal of a lesion
What is an excisional biopsy?
I am considered the powerhouse of the cell
What is mitochondria?
I am the commonly used fixative in a Histopathology Laboratory.
What is 10% Formalin?
Goals of decalcification
What is complete removal of calcium salts?
What is minimal distortion of cell morphology?
What is no interference with staining?
I am a universal solvent. No need for dehydration and clearing when I can do it all.
What is dioxane, tertiary butanol + tetrahydrofuran?
Removal of pleural fluid
What is Thoracentesis?
Process in which the entire cell is destroyed by its lysosome is
For adequate fixation of tissues, the appropriate fixative volume should be
What is 15-20x greater than the tissue volume?
I am a criteria for specimen rejection.
What is mismatched biographical information?
What is unlabelled specimen?
What is unfixed specimen?
Paraffin wax during infiltration is set at this temperature.
What is 58 to 60°C?
Removal of synovial fluid from joints
What is arthrocentesis?
What is active cell?
Formalin pigment can obscure cell details, therefore, this reagent can be used to remove formalin pigments from tissue sections.
What is Alcoholic picric acid? or What is Alkaline alcohol?
Grossing is an important process in Histotechnology. You ought to take note of key features of tissues. These key features include
What is size, colour, consistency, texture, and any abnormalities?
Prolong heating of tissues in paraffin wax during infiltration will lead to
Quality control that I can employ when embedding tissues.
What is opening one cassette at a time?
What is wiping forceps after handling each tissues?
What is monitoring of temp of station?
What is confirming # of cassettes + tissues?
DNA replication occurs in this stage of the cell cycle
What is S phase?
Picric acid is present in Bouin solution, when used, if excess picric acid remains in embedded tissues, the consequence is
What is the stain will deteriorate over time?
I am called dystrophic calcification. Therefore, my characteristics include:
What is normal serum calcium level?
My name is Benzene, if you're in contact with me for a prolonged period of time, over the years, you'll be at risk of
What is blood/bone marrow (leukaemia) disorder?
The purpose of embedding tissues in Histotechnology
What is to provide external support to the infiltrated tissues?
What is to orient the tissue to ensure adequate histology will be seen?
My major role is in detoxification and I am abundantly found in the liver.
What is smooth endoplasmic reticulum?