Show:
Real-Time PCR
Stem Cells
Stem Cell Characterization / Cell Analysis
Sequencing
100
Is SYBR Green detected by the instrument when DNA products are denatured?
No
100
What are two functional characteristics of stem cells?
infinite self-renewal (can make copies of themselves forever), ability to differentiate (turn into) other, more specialized cells (e.g., liver, blood, brain)
100
The International Stem Cell Initiative (ISCI) suggested a core set of markers that are consistently expressed across multiple human embryonic stem cell lines. Name one of those markers.
Nanog, TRA-1-60, TRA-1-81, SSEA4, SSEA3, Sox2, TDGF, OCT4, GABRB3, GDF3, or DNMT3B
100
What is the maximum read length that can be done on the S5?
600 bp
200
Of the 3 phases of PCR which phase provides precise and accurate quantification?
Geometric phase or exponential phase
200
What are the three main categories for stem cell applications?
Regenerative Medicine, Drug Discovery/Development, Developmental Biology
200
Old cytometers use fluid to focus cells, what does the Attune use?
Acoustic Focusing
200
What is a barcode?
Molecular identifier used for multiplexing samples
300
What are the 2 principles that are required for a TaqMan assay?
FRET and Exonuclease activity of Taq DNA polymerase
300
Name at least one type of morphological feature when looking at a stem cell culture under the microscope.
Compact colonies, Defined/refractive borders, large nuclei
300
The Scorecard allows you to determine both the pluripotency and what other characteristic of your stem cell sample being analyzed?
Differentiation (into ectoderm, endoderm, and/or mesoderm)
300
What three processes does the Ion Chef complete?
emPCR, enrichment, and chip loading
400
How do you determine the efficiency of a real time PCR assay?
Run a standard curve
400
What is the name of the newest feeder-free media system for culturing human pluripotent stem cells that we used in the lab exercises?
StemFlex
400
What is the main reason a flow cytometer will need service?
Laser Alignment
400
How do the Ion platforms determine if a base has been incorporated?
Detecting the pH change cause by the Hydrogen Ion release
500
Can we start with RNA as our template in a real time reaction run? Why?
No. DNA or cDNA must be the starting material.
500
What ion does EDTA chelate in order to loosen the cell-cell bonds in stem cell cultures?
Calcium ions
500
In flow cytometry what light scatter property is directly related to the size of the cell being analyzed?
Forward Scatter
500
What is the data analysis workflow? 
Raw data acquisition, signal processing, basecalling, filtering/trimming.