Diet Design
Which component of the diet was systematically varied across treatment groups?
Lipid (fat) level
Which lipid level range resulted in the best growth performance?
Moderate lipid levels (~79.6 – 129.1 g/kg)
Which marker was used to measure lipid peroxidation in this study?
MDA
What do caspase-3 levels indicate?
Apoptosis (cell death/damage)
Why is increasing dietary lipid not always beneficial, despite increasing energy availability?
Because excess lipid leads to oxidative stress, inflammation, and impaired health despite higher energy
Why were the diets formulated to be isonitrogenous?
To keep protein levels constant, so any effects observed are due to lipid levels
Name two growth parameters that declined at high lipid levels
Weight gain (WG), Specific growth rate (SGR) or feed efficiency (FE)
Why does high lipid intake lead to oxidative stress?
Excess fatty acid oxidation overloads mitochondria, increasing ROS production
What does the simultaneous upregulation of ppara and acc suggest about lipid metabolism?
An imbalance where both lipid oxidation and synthesis are increased