Manipulation of gene expression in prokaryotes

yellow

100

The direct manipulation of an organism’s genome

Genetic engineering

200

Joins vector and gene to be cloned

Ligase

300

Give me one example of Genetic engineering applications

Production of recombinant vaccines.

red

100

An organism that is generated through genetic engineering.

GMO (Genetically Modified Organism)

200

Cuts DNA at specific sequences

Restriction enzyme

Blue

100

Only contains coding regions or exons

cDNA

200

Produces amplification of target DNA

PCR (Polymerase Chain Reaction)

300

Good cloning vector (why: small, easy to manipulate, replicates independently).

Plasmid 

purple

100

To isolate large quantities of specific genes in pure form.

Gene cloning purpose

200

Transfer of genetic material from one bacterial cell to another by a bacteriophage.

Transduction

300

Can hold larger amounts of DNA than most plasmids

Lambda phage

green

100

Incorporation of free DNA into a recipient cell bringing genetic change.

Genetic transformation

200

Physical transfection method using an electrical pulse to create temporary pores in cell membranes.

Electroporation

300

Plants genetically engineered with one or more genes from another species.

Transgenic plants