Solutions
Number of EMEM barrels used for a full week of production.
How many is 4 barrels of EMEM?
The acronym MGM stands for this.
What is Marek's Growth Media?
This many amps are used per syringe.
How much is 25 amps?
At Harvest, the "Pouring" Carboy is filled up to this many Liters. (before the final Carboy)
How much is 10L?
350,000mL MGM and 12,467mL cells are used to plant 1200 roller bottles, what is the actual amount of media per bottle?
(350000mL MGM + 12467mL cells) / 1200 RB
(362467mL) / 1200 RB
302.05 mL/RB
≈302 mL/RB
Total amount of Cryo made on Wednesday for Batch on Thursday & Friday.
How much is 14L of Cryo?
(7L Thursday + 7L Friday)
The pumps are calibrated to this amount.
How much is 300mL?
This many Control Bottles are needed.
How much is 4 Control Bottles?
The number of Newborn Solution bottles recorded on the Harvest Cart Sheet.
How many is 8 NBS bottles?
1200 roller bottles are expected to be planted for CEF Lot 269 X, on Monday. 4 empty bottles are dropped during racking. 1 planted roller bottle was not closed all the way and emptied during the night. 2 inoculated bottles fly off the table during Tuesday’s Inoculation. The rest of the bottles surprisingly survive all of Harvest. How many bottles were Harvested on Thursday?
(1200 planted RB - 1 planted RB - 4 control RB - 2 inoculated RB)
1193 harvested RB
This raw material needs to be gently swirled, before added to the MGM mixture.
What is L-Glutamine?
This is the amount of MGM, in milliliters, used per plant.
How much is 350,000mL MGM?
The earliest time Inoculation can start is __ but the ideal time to start is __.
When is 15:30 and 16:00?
The Trypsin station pump is calibrated to this many milliliters and the Harvest racks are supposed to spin Trypsinized roller bottles for this many minutes.
How much is 70mL and how long is 5 minutes?
195 amps, of Seed Lot 10283-202, are thawed for Resuspension. 1 amp brakes in the mouse cage. The rest of the amps are added to 10L of MGM. 1196 roller bottles are expected to be inoculated. What should the pumps be calibrated to?
[((195 amps - 1 dropped amp)x2.1mL) + 10000mL MGM] / 1196 RB
((1194)x2.1mL) + 10000mL MGM) / 1196 RB
(407.4mL + 10000mL MGM) / 1196 RB
(10407.4 mL Inoculum) / 1196 RB
8.7 mL/RB
The amount of IFCS bottles recorded on the "MGM Cart Sheet" to make 4 barrels of EMEM into MGM.
How much is 44 IFCS bottles?
Monday's heat room is __ & Tuesday's heat room is __.
What is 544 on Monday and 543 on Tuesday?
These items come in the Resuspension/Inoculation "dish kit".
What is 1 Mouse Cage, 1 Stir Bar, 1 Inoculation Tube Cap, 2 Inoculum Bells, and 1 500 mL Cylinder?
The "Large/New" centrifuge holds __L and the "Small/Old" centrifuge holds __L.
How much is 10L and 6L?
What is the age of the monolayer, if 1023-2023-CEF-296-A was planted, at start time and date of 12:37 on 01Apr23 and the inoculation start time, of Antigen 283-2562, is 15:30 on 02Apr23?
(12:37 01Apr23 → 12:37 02Apr23 = 24 hours) + (12:37 02Apr23 → 15:30 02Apr23 ≈ 3 hours)
24 hours + 3 hours
27 hours
1 barrel of MGM consists of __mL IFCS, __mL L-Glutamine, __mL Amphotericin, __mL Gentamicin, and __mL EMEM.
How much is 10,800mL IFCS, 1,800mL L-Glut, 150mL Ampho, 216mL Gent, and 167000mL EMEM?
The acronym EMEM stands for this.
What is Eagle's Minimal Essential Medium?
The expected MOI per roller bottle.
What is 0.006 MOI/roller bottle?
The 3 basic settings for each centrifuge.
What is Speed (2000 rpm), Temperature (4°C), and Time (13 minutes)?
For Antigen 283-2544, 175 amps were thawed, for 1196 roller bottles. 100 amps were thawed from Seed Lot 10283-167 and 75 amps were thawed from Seed Lot 10283-170. Seed Lot 10283-167 had a titer of 2.7777x10^6 pfu and Seed Lot 10283-170 had a titer of 2.7414x10^6 pfu. All amps were added to the inoculum. The pumps are calibrated for 8.7mL, to be added per planted roller bottle. 3 infected roller bottles fell off of the table, during Inoculation. 1197 roller bottles were placed into the heat room. The actual amount of Inoculum added to each roller bottle was 8.7mL. What is the Actual pfu/roller bottle?
[(H1xA1) + (H2xA2)] / (C + D1) / (D2/B)
[(2.7777x10^6 x 100) + (2.7414x10^6 x 75)] / (3 + 1193) / (8.7/8.7)
[(2.7777x10^8) + (2.0560x10^8)] / (1196) / (1)
404,159.699
4.0x10^5 pfu/RB