Nuclear Decay
A nuclear reaction that yields a daughter atom with a mass # 4 less and an atomic # 2 less than the parent.
What is Alpha Decay?
This type of inhibitor will increase the Km of a given reaction while leaving Vmax unchanged at high substrate concentrations.
What is a Competitive Inhibitor?
A bio lab method that seperates vaporized molecules based on affinity.
What is Gas Chromatography?
A type of restriction enzyme that targets modified (e.g. methylated, hydroxymethylated) DNA.
What are Type IV REs?
Positively charged species that "want" more electrons.
What are Electrophiles?
In this nuclear reaction, a positron is emitted from the parent nucleus and the daughter has one less proton.
What is Beta Plus Decay (positron emission)?
This type of inhibitor will lower Vmax but leave Km unchanged.
What is a Noncompetetive Inhibitor?
A bio lab method used to measure the size of one molecule through ionization.
What is Mass Spectrometry?
A type of restriction enzyme that cleaves at short distances from their recognition sites and require magnesium.
What are Type II REs?
A species with an excess of electrons and that "wants" to use them to form bonds.
What are Nucleophiles?
In this nuclear reaction, a neutron is converted into a proton and an electron is emitted. The daughter has an atomic number that increases by 1.
What is Beta Minus Decay?
This type of inhibitor will lower both Vmax and Km.
What is an Uncompetetive Inhibitor?
A bio lab method that seperates molecules based on affinity using a liquid mobile phase for seperation.
What is Thin-Layer Chromatography?
A type of restriction enzyme that cleaves far from the recognition site and requires both ATP and S-adenosyl-L-methionine to function.
What are Type I REs?
This event occurs when a nucleophile uses excess electrons, either from a free pair or pi/sigma bonds, to bond with an electrophile.
What is a Nucleophilic Attack?
In this nuclear reaction, an excited nucleus releases energy as a highly powered photon yielding the same element with an identical daughter atom, in a more stable, lower energy state.
What is Gamma Decay?
The substrate concentration required to get to half of the maximum velocity reached by the reaction.
What is Km?
A bio lab method used to identify amino acids in a novel protein.
What is Edman Degredation?
A type of restriction enzyme that cleaves at short distances from their recognition sites and requires ATP.
What are Type III REs?
In an organic extraction, these compounds move to the organic layer.
In this nuclear reaction, a nucleus "grabs" an electron, which changes a proton into a neutron, leading to the same mass # but -1 atomic #.
What is Electron Capture?
The maximum velocity reached by an enzyme kinetic reaction.
What is Vmax?
A bio lab method used to study protein-protein interactions.
What is Co-immunoprecipitation (Co-IP)?
These form when a restriction enzyme doesn't cleave at the same nucleotide on both strands.
What are Sticky Ends?
What is a Ketone?