Lab Math
How many uM is 30nM?
0.03uM
A volume of 10um is most appropriately pipetted with what pipette?
p20
What cells do we use in this lab?
Tetrahymena thermophila
What templates do we use for PCRs in this lab?
cDNA and gDNA
What does the brightness of a band on a gel following a PCR tell you?
Relative amount of PCR product
40kg is how many g?
40,000g
On a pipette to which stop do you go to suck up liquid?
First
Hydroxyurea damages what in cells?
DNA
What does PCR stand for?
Polymerase Chain Reaction
Housekeeping genes are what type of control?
Negative
How many grams of salt go into 1L of water to make a 0.9% saline solution?
9g
A p1000 set with the following numbers is ___ : 1,2,5.
Broken
The two main DNA damage repair pathways are NHEJ and what?
Homologous Recombination
In gel electrophoresis, DNA moves towards which charged end of the gel?
Positive
Having no bands or ladder on a gel indicates that what reagent may have not been added to the gel?
SybrSafe
To make 50mL of a 0.1X solution you need ___mL of a 1X stock solution.
5mL
When focusing a microscope, its best to bring the slide ___ to the objective lens and slowly move it.
Close
What protein can convert RNA into cDNA?
Reverse Transcriptase
What are the three stages of PCR?
Denaturation; Annealing; Elongation
In this experiment, ___ is the positive control.
Rad51
How much of a 70% stock solution do you need to make 100mL of a 3.5% solution?
5mL
When cell counting, cells on the ___ and ___ borders are counted.
Top or Bottom; Left or Right
Dicer binds to what complex to cut sRNAs?
RISC complex
What is the temperature for elongation in PCR?
72C
Why could your gel have a ladder but no bands?
Several correct answers