Introduction to Biochemistry
Amino Acids & Proteins
Enzymes
Inhibition
Immunity
100
The universal solvent is
What is water?
100
What is the general amino acid structure?
Amino group, carboxyl group, and side chain
100
Do enzymes change ΔG
No
100
In competitive inhibition, the inhibitor occupies the...
Enzyme active site
100
Which part of the immune system is the first line of defense?
The innate/nonspecifc immune system (skin, respiratory system, GI tract, etc)
200
The hydrogen-bond acceptor in H2O
What is Oxygen?
200
Which amino acid is represented by F
Phenylalanine
200
What is Km?
Km is the concentration of substrate at which 50% of enzyme active sites are occupied by substate. (Affinity)
200
How does uncompetitive inhibition affect Km and Vmax
Decreases both Km and Vmax
200
What are the 2 types of lymphocytes and where do they mature?
B cells: bone marrow
T cells: Thymus
300
The Ka formula of this equation is:
CH3COOH(aq) + H2O(l) ---> H30+(aq) + CH3OO-(aq)
What is:
Ka = [H3O+][CH3COO-]
[CH3COOH]
300
Which structure are disulfide bridges formed and how?
Tertiary structure of a protein by the oxidation of two cysteine residuals
300
What graph does this represent and what is the equation?
The Michaelis-Menton graph
300
Which type of inhibition is represented by these graphs
Competitive inhibition
300
What do B cells and T cells do?
B cells: Produce antibodies
T cells: Destroys pathogens
400
Out of these examples, which choice(s) can be considered a buffer solution?
A) 1M CH3CO2H/NaCH3CO2
B) 1M HCl/NaCl
C) 1M HF/ NaF
What is A and C?
400
What is a conjugated protein?
A protein that has a prosthetic group important for protein function.
400
What does the x-intercept and y-intercept represent on the Line-Weaver Burke graph?
X-intercept: -1/Km
Y-intercept: 1/Vmax
400
Which inhibition is represented by this graph
Noncompetitive inhibition
400
How do vaccines work?
They trigger the immune response and create memory B and T cells for future infection
500
The Henderson-Hasselbach equation is:
What is:
pH = pKa + log [Conjugate base]
[Weak acid]
500
Which 2 amino acids do not have L-configuration
Glycine & Cysteine
500
Which class of enzymes catalyze redox reactions?
Oxidoreductases
500
What happens when a substrate binds to an allosteric enzyme?
Stimulates/inhibits the binding of other substrate molecules (cooperative kinetics)
500
Which MHC receptors do CD4 and CD8 T cells recognize?
CH4: MHC 2
CD8: MHC 1