Aerobic Plate Count
This agar is used for enumerating bacteria in food samples during APC.
What is Tryptone Glucose Yeast Extract (TGY) agar?
This type of agar is used to differentiate B. cereus by its resistance to Polymyxin B, mannitol fermentation, and lecithinase activity.
What is MYP agar?
The preferred culture medium for isolating Coagulase Positive Staphylococci in this method.
What is Baird-Parker agar?
This nutrient medium is used first to resuscitate stressed cells before adding the VRBA overlay.
What is Trypticase Soy Agar (TSA)?
This is the minimum and maximum colony count range for selecting yeast and mold plates.
What is 10 to 150 colonies?
What is the standard incubation time and temperature for typical food samples in the APC method?
What is 35°C for 48 hours?
What do suspect B. cereus colonies on MYP agar look like?
What is eosin pink and are usually large in size. The surface of the colony will appear grainy, dull or like frosted glass.
Colonies that lack the opaque or clear zones are described as this.
What are atypical colonies?
This type of plate is used to confirm colony morphology before biochemical testing.
What is an EMB (Eosin Methylene Blue) plate?
This type of medium is used with antibiotics to suppress bacterial growth while culturing fungi.
What is Potato Dextrose Agar (PDA) with antibiotics?
What is the optimal colony range per plate for accurate counting?
What is 25 to 250 colonies?
This step is recommended if colony reactions are unclear after 24 hours.
What is incubate for an additional 24 ± 2 hours?
The characteristic color of typical Staphylococcus aureus colonies on Baird-Parker agar.
What is black or jet-black with a halo or clear zone?
Coliforms are used as this type of organism in food testing.
What are indicator organisms?
This solution can be used as an alternative to antibiotics upon client request.
What is tartaric acid solution?
What does TNTC stand for on a colony count record?
What is Too Numerous To Count?
This chromogenic agar is an alternative to MYP that better inhibits background flora.
What is BACARA?
The reaction score in the coagulase test that indicates a strong positive result.
The reaction score in the coagulase test that indicates a strong positive result.
This is the fermentation broth used to confirm coliforms.
What is Brilliant Green Lactose Bile (BGLB) broth?
These two antibiotics are commonly added to PDA to inhibit bacteria.
What are chloramphenicol and chlortetracycline?
How should plates be stacked during agar pouring and during incubation to avoid recovery issues?
What is no more than 10 during pouring and no more than 12 during incubation?
This chemical indicator is used in MYP to show acid production from mannitol fermentation.
What is phenol red?
This coagulase test reagent is derived from an animal and requires rehydration before use.
What is rabbit plasma with EDTA?
Typical E. coli will test positive for these two IMViC tests.
What are Indole and Methyl Red?
The purpose of adding antibiotics to PDA instead of acidifying it.
What is to avoid stress on yeasts and molds and allow better recovery?