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Gel Electrophoresis
Metric Conversions
DNA
Solutions & Dilutions
Hodge Podge
100
This pushes macromolecules like DNA through a gel.
What is an electric current?
100
The prefix u represents this much in scientific notation.
What is 10^-6?
100
DNA is composed of this many different nucleotides.
What is 4?
100
A homogenous mixture.
What is a solution?
100
GLP
What is Good Laboratory Practices?
200
Gel electrophoresis separates molecules based on these two characteristics.
What is size and charge?
200
120uL is the same as this many mL.
What is 0.120 mL?
200
These are the three components of a nucleotide.
What are phosphate, deoxyribose (or sugar), and a nitrogen base?
200
This component is increased to make a solution more concentrated.
What is the solute?
200
GMP.
What is Good Manufacturing Practices?
300
DNA separates in gel electrophoresis based on this characteristic.
What is size?
300
980 nm is the same as ______ cm.
What 0.0000980 cm?
300
These types of bonds hold base pairs together.
What are hydrogen bonds?
300
The dilution equation.
What is C1V1=C2V2?
300
6.91 uL is this in scientific notation.
What is 6.91 x 10^-6?
400
This helps track the movement of the samples through the gel.
What is a loading dye?
400
398 kg converted to mg is. . .
What is 398 000 000 mg?
400
Purines.
What are adenine and guanine?
400
This amount of agarose powder is needed to make a 70mL gel that is 0.8% m/v.
What is a 0.56 g of agarose?
400
The amount of a specified substance in a given amount of another substance is the. . .
What is concentration?
500
These restriction fragments would migrate the furthest.
What are small(est) fragments?
500
The metric system changes by this factor.
What is 10?
500
This base pair forms two hydrogen bonds between the bases.
What is thymine and adenine?
500
This volume of 9.0M TAE buffer is needed to make 100 mL of 0.5M TAE buffer.
What 5.6 mL?
500
The negative charge of DNA is due to this component of a nucleotide.
What is the phosphate group?