ELISA
ELISA/Serial Dilution
Cycle Sequencing
Diagnosis
Other
100
Which of the following cannot be determined by an ELISA test?
a. Concentration of antibodies or antigens that are the target of the test
b. Presence of disease
c. Genetic sequence of a pathogen
d. Who was likely the first person infected in the outbreak
C
100
Why do you need a positive and negative control when doing an ELISA?
To see whether your results got contaminated
100
After we determine the DNA sequence, what is the name of the tool used to identify the pathogen?
BLAST
100
Which patients have meningitis?
John, Jessica, Emmanuel
100
What is a medical intervention?
Anything that improves health or alters the course of a disease
200
What would happen if your group forgot to add the secondary antibody before adding the substrate?
There would be no color change since the enzyme is attached to the secondary antibody.
200
What is the purpose of doing a serial dilution?
To quantify the amount of color change that occurred
200
What is the purpose of doing electrophoresis in the capillary tube?
We separate the fragments by size so we can analyze which order the nucleotides go in
200
Which patient was likely infected FIRST?
Jessica
200
What college did Mrs. Lauzon go to?
South Dakota School of Mines and Technology
300
What would happen if your group forgot to wash out the secondary antibody before adding the substrate?
Every well would change color because the enzyme would be present in every well
300
What is box C?
1/5
300
How do we make sure we are sequencing pathogen DNA and not human DNA?
We do PCR using primers that are specific to genes that only bacteria and viruses have
300
Joselyn has a high fever, a headache, and a sore throat. She is convinced that she caught meningitis from her friend Jessica, and she wants you to prescribe antibiotics for her. What should you do?
Do not prescribe her antibiotics - she tested negative for meningitis. You should run tests to determine what she's sick with.
300
What is the relationship between antigens and antibodies?
antibodies bind to antigens
400
What is the correct order for the ELISA test?
antigen/sample, primary antibody, secondary antibody, substrate
400
What is box I?
100 ug/uL
400
What are two purposes of the fluorescent nucleotides?
They act like a stop sign, ensuring that every fragment is a different length. They are color coded which makes it possible to identify which nucleotide is at the end of each fragment
400
What concentration of antigen does Emmanuel have?
500 ng/uL
400
Your brain is constantly "eating itself" to change it's structure. This occurs when cells envelop and consume smaller cells/molecules. What is this process called?
Phagocytosis!
500
What does ELISA stand for?
Enzyme linked immunosorbent assay
500
What is box F?
1/40
500
ATTATTCAGTAGTGCT
500
What are five diseases that show symptoms of persistent fever, sore throat, headaches, and fatigue?
meningitis, COVID, flu, mono, strep throat
500
What is the range of teeth a snail can have?
Between 1,000-12,000!