Stains
Gram's iodine is a ____ used during the _____ stain
mordant, gram
True or False: Yeast can only reproduce through budding.
False
True or False: Mannitol-salt agar, KF Strep agar, EMB agar, and LST MUG broth are selective and differential
True
True or False: S. salivarius colonies appear blue and “gum drop” shaped on mitis-salvarius agar.
True
Safranin is used to stain Gram __________ bacteria
negative
True or False: Yeasts and bacteria are eukaryotic microbes
False.
LST-MUG detects ____ and ____.
fecal coliforms AND coliforms
True or False: Turbidity, gas, and fluorescence in LST-MUG broth and growth on EMB agar means your sample is positive for E. coli.
False; colonies must also have a metallic green sheen on EMB agar to be considered positive for E. coli
What stain is Bacillus subtilis the control culture?
Endospore stain
What mold structure contain reproductive spores like sporangiospores and conidiospores
aerial hyphae
Mannitol-Salts broth contains what 2 differential ingredients?
mannitol and phenol red
You streak a Mannitol-salt agar plate and after incubation you see growth and the agar around the growth has turned yellow. What bacteria was inoculated?
Staphylococcus aureus
The positive control for the Gram stain is ______ and is stained by _______ dye.
Staphylococcus epidermidis, crystal violet
What 2 characteristics of SAB agar support the growth of molds and inhibit bacterial growth?
pH 5-6 and high sugar content
Triphenyltetrazolium Chloride is differential for ______ and is in _______ agar.
anaerobic metabolism, KF-Strep
LST MUG you see growth and gas. What would you expect to see on the gram and endospore stain?
Gram negative, vegetative cells only (no endospores)
You are handed a culture of Klebsiella pneumoniae, what stain would you most likely perform? What dyes are required to perform this stain?
Capsule stain with Congo Red and Maneval’s solution
What are the 2 yogurt starter cultures?
Streptococcus thermophilus and Lactobacillus bulgaricus
Methylene blue is ______for ______ bacteria and is a component of _____ agar.
selective, gram negative, eosin methylene blue
You are given a bacterium that is Gram positive. You streak a mitis-salvarius plate. After incubation, there are small, flat, black bacteria on the mitis salvarius agar. What bacteria were you given?
Enterococcus