Micro: Bacteriology

Bacteria Structure

100

Used to translate genetic material into proteins

Ribosome 

200

Unbonded region where DNA is found 

Nucleoid

300

Sticky outer layer that aids in antibiotic resistance 

Capsule/Glycocalyx

400

Provides structure and is directly responsible for gram staining result 

Cell Wall

500

Long structure used for propelling bacteria through medium. Common in Bacilli structures 

Flagella

Bacteria Staining

100

Stain that yields a positive or negative result based on the thickness of a cellular structure. 

Gram staining 

200

Name the three shapes 

1. Spherical 

2. Rod

3. Spirals

1. Cocci

2. Bacilli

3. Spirilla 

300

Secondary staining that must be used if the cell has a hydrophobic layer outside the cell wall

Acid-Fast

400

Result given to pink bacteria past gram staining 

Gram negative 

500

Illness caused by a bacterium with a waxy outer layer 

TB

Growing and Testing

100

Technique used to eliminate contamination from pathogens 

Aseptic

200

1. Introducing the specimen to a growth medium 

2. Microorganisms that have grown/process of growing

1. Inoculation

2. Culture 

300

Media that favors some organisms while suppressing others 

Selective 

400

Media that identifies bacteria based on reactions in the media 

Differential 

500

This bacteria thrives on salt agar and produces a yellow ring around the colonies

Staph 

Susceptibility Testing

100

susceptibility test that first helps determine antibiotic resistance 

Disk Diffusion

200

What does a clear ring around an antibiotic disk mean in disk diffusion

The bacteria is susceptible to that antibiotic 

300

What does broth dilution test for?

The minimal inhibitory concentration (MIC)

400

1. The minimum concentration of antibiotic that produces no VISIBLE growth 

2. The minimum centration of antibiotic that produces NO growth 

1. Minimum Inhibitory Concentration

2. Minimum Bactericidal Concentration

500

After broth dilution and the MIC is determined, what happens next to determine the MBC

Culturing to find the Minimal Bactericidal Concentration (MBC)

Bacterial Diseases

100

What are the two primary susceptibility tests? 

Disk Diffusion and Broth Dilution

200

A microorganism capable of causing disease

Pathogen

300

Feature of this bacteria that prevent effective gram staining

Mycolic acid/Waxy envelope

400

Staph infection that is resistant to methicillin. How do we determine resistance? 

MRSA; disk diffusion

500

Why would you not want to kill all E.Coli bacteria when you have a pathogenic E.Coli infection?

E. Coli is part of the normal flora. So, killing all E. Coli could make you sicker.