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Replication
Transcription
Translation
Gene Regulation
Gene Transfer
100

During replication, what is the key enzyme which synthesizes new DNA?

DNA polymerase.

Will not take just polymerase!

100

Please list all of the subunits of the RNA polymerase holoenzyme.

a, a, b, b', sigma

100

The shine delgarno is a binding site for _____

ribosome

100

What specific sigma factor is required to activate housekeeping genes?

alpha

100

What modification do bacteria make to their DNA to protect against restriction enzymes?

DNA methylation

200

During the initiation of replication, DnaA binds to what region of the chromosome?

OriC

Also accept origin of replication

200

Where on the DNA does the sigma factor bind in relation to the start of the gene?

-35 and -10

200

aug is the RNA transcript for a start codon.  What amino acid does this code for?

formyl-methionine

200

Operators are location on the DNA where _____ bind.

Repressors

200

What class of genetic transfer utilizes the tra machinery?

Conjugation

300

DNA is synthesized in which direction?

5' to 3'

300
What method of transcription termination requires the use of ATP

Rho-dependent

300

The second tRNA delivered to the ribosome is delivered by______?

EF-tu

300

Two-component systems are made of a histidine protein kinase (HPK) and a _______

response regulator

300

During transformation, what is done to the DNA before it enters the cell?

Made into single strand DNA

400

What protein is responsible for the decatenization of newly replicated chromosomes?  What separates the two rings?

Topoisomerase IV

Will take just Topoisomerase

400

In which direction is mRNA synthesized?

5' to 3'

400
New amino acids are added to what side of a polypeptide chain?

C-term

400

In the lac operon, activated CAP acts as a ______.

activator

400

What is the term for a F+ bacterial cell that has integrated the f factor plasmid into it's own genome?

Hfr strain

500

What protein is responsible for the termination of replication?

Tus

500

What 2 RNA features are required for rho-independent transcription termination?

Hairpin/loop, and polyU tail.

PolyA tail is the feature of DNA, but will count for today. (not on the exam though!)

500

Which antibiotic blocks the translocation of the ribosome?

erythromycin

500

cAMP is produced by what protein?

adenylate cyclase

500

during transfer of the f-factor plasmid, what specific type of plasmid replication occurs?

Rolling circle