Microscopy
Notable Scientists
Stains and Slide Prep
Microbes
Soil (TE section 2)
100

The range of light waves that can be seen by the human eye.

What is the visible spectrum? 

100

This Greek scientist is known as the "Father of Western Medicine".

Who is Hippocrates

100

Stains are used to view microbes with light microscopes because otherwise, most microbes would be this.

What is invisible/clear?

100

These prokaryotes can be found everywhere.

What are bacteria?

100

This layer of soil, rich in organic matter and containing most soil life, is also known as topsoil.

What is the A Horizon?

200

A type of microscope that has both and ocular lens and an objective lens.

What is a compound microscope?

200

This "Father of Microbiology" first described tiny creatures he saw through magnification as "animalcules".

Who is Leeuwenhoek? 

200

This stain allows microbes to be viewed alive and allows a clearer view of the shape of the microbes.

What is negative stain?

200

These common acellular pathogens do not meet the requirements to be considered alive.

What are viruses?

200

This layer of soil contains lower levels of microbial life and a greater proportion of anaerobic bacteria.

What is B Horizon?

300

This lens shape allows images/objects to appear magnified.

What is convex? 

300

This scientist first proposed that it is things that we cannot see that cause disease.

Who is Varro?

300

Process by which a flame or hot plate is used to kill and make a specimen "stick" to the slide.

What is heat fixing?

300

Name 3 of the shapes of bacteria.

What are coccus, bacillus, coccobacillus, vibrio, spirochete? 

300

Antibiotics are a type of these compounds that are produced by bacteria that enhance their chances for survival, while remaining nonessential for fundamental growth processes. 

What are secondary metabolites? 

400

This instrument uses subatomic particles to allow up to a 100,000x magnification.

What is an electron microscope? 

400

This French chemist disproved spontaneous generation.

Who is Louis Pasteur?

400
3 most commonly used dyes for a simple stain.

What are crystal violet, blue methylene, and safranin?

400

These microbes can be unicellular or multicellular and have cell walls made of chitin.

What are fungi? 

400

This is the name for the part of the soil that is in direct contact with living plant roots and their secretions. Everything else is "bulk soil".

What is the rhizosphere? 

500

This liquid is used when viewing specimens on the highest magnification setting in order to improve resolution.

What is immersion oil? 

500

This scientist died in an asylum after proposing handwashing between procedures (autopsies and childbirth) as a means of preventing maternal mortality. (This reduced postnatal mortality by 90%)

Who is Semmelweis?

500

This stain differentiates between bacteria that have different types (thicknesses?) of cell walls.

What is Gram stain? 

500

These cellular structures are found in both prokaryotes and eukaryotes. (4)

What are the plasma membrane, cytoplasm, ribosomes, and chromosomes?

500

The physical structure of the soil is determined by the proportions of these 3 main particle types.

What are sand, silt, and clay?