Requirements
for
Growth
for
Growth
How does bacteria reproduce?
By splitting in half
What natural compounds can break down Biofilm? (More than one)
A. Vitamin C
B. Essential Oils
C. Alcohol
D. Food Based Supplement
A. Vitamin C
B. Essential Oils
D. Food Based Supplement
What type of blood is usually used in Blood Agar?
A. Human Blood
B. Fish Blood
C. Mammalian Blood
D. Reptile Blood
C. Mammalian Blood
What is the percentage Chocolate in Chocolate Agar?
0%
Chocolate Agar contains NO CHOCOLATE
In which phase of the bacterial growth curve does growth come to a halt?
During the stationary phase the population grows slowly or stops growing because of decreasing food, increasing waste, and lack of space.
What are the main requirements for bacterial growth?
Who is the father of microbiology?
A. Thomas Edison
B. Claude ZoBell
C. Van Leeuwenhoek
D. Sheldon J. Plankton
C. Van Leeuwenhoek
Which Special Media Category goes Chocolate Agar fall into?
A. Enrichment Media
B. Selective Media
C. Differential Media
A. Enrichment Media
What is the main difference between Differential and Selective Media?
Selective Media contains ingredients that kill unwanted bacteria growth. Differential media takes advantage of bio chemical properties of target organisms, leading to visible change when growth is present.
What is the longest stage in the bacterial growth curve?
A. Lag Phase
B. Exponential Phase
C. Stationary Phase
D. Death Phase
D. Death Phase
What does the acronym F-A-T-T-O-M stand for?
F- ood
A-cidity
T-ime
T-emperature
O-xygen
M-oisture
True or False
Biofilms are not dangerous and do not attach on the human body.
False
Biofilms cause a variety of diseases and are dangerous.
Which type of media contains factors that give colonies distinct, recognizable characteristics?
A. Selective Media
B. Differential Media
C. Enrichment Media
B. Differential Media
What are the three main types of culture media?
Enriched Media
Selective Media
Differential Media
Which factors influence Binary Fission?
Ph + Temperature + Oxygens
True or False
Bacteria can reproduce and grow out in the environment.
True
What are biofilms?
Biofilms are aggregates of one or more species of microbes, adhered to a surface, which work together to survive.
What is Culture Media?
Culture media is a gel or liquid that contains nutrients and is used to grow bacteria or microorganisms.
Which enrichment media contains heated blood?
A. Blood Agar
B. Chocolate Agar
Chocolate
What is Microbial Growth?
The term microbial growth refers to the growth of a population (or an increase in the number of cells), not to an increase in the size of the individual cell. Cell division leads to the growth of cells in the population.
If there is 5 bacterial present to begin with and 20 bacteria present at 30 minutes, how many will be present at 60 minutes? (hint: this is the example from the video)
60 min = 80 bacteria
Name 3 triggers for biofilm formation.
Quorum sensing
Hostile environmental conditions
Nutrient availability
Hydrodynamic conditions
Which category does MacConkey Agar fall into?
A. Selective Media
B. Differential Media
C. Selective & Differential Media
D. Enrichment Media
C. Selective & Differential Media
What is a nutrient Agar or Broth?
Peptone meat extract tryptic water
Put the Four Stages of Bacterial Growth in Chronological Order.
-Exponential Phase
-Death Phase
-Lag Phase
-Stationary Phase
1. Lag Phase 2. Exponential Phase 3. Stationary Phase 4. Death Phase