Cleaning
What do we call this piece of glass in a germicidal wipe test?
Carrier
What kind of product is Renew Life?
Probiotic
What are the two types of food testing?
Preservative and Pathogen
What strain of Mycobacterium does the micro lab use to represent M.tuburculosis?
M.bovis
What type of spore forming bacteria does the micro lab use for testing?
C. difficile
What is the most common bacteria to use in a germicidal test because it is considered hard to kill?
S. aureus (Staph)
What are the two classes of probiotics enumerated for Renew Life?
Lactobacillus and Bifidobacterium
What is the laboratory method used to prevent contamination of pure cultures, sterile equipment and the environment?
aseptic technique
What are the two methods of growing M.bovis?
Static vs Shaken
In what machine are C.difficile plates incubated in?
Anaerobic Chamber
What is the standard incubation temperature of E.coli?
36 degrees Celsius
What is Water Activity?
free or non-chemically bound water in a substance (Aw)
What are common bacteria used in Food Pathogen testing?
E.coli, Listeria, Salmonella
What type of staining is used to visualize M.bovis under a microscope?
Acid Fast (waxy coating prevents gram staining)
What gasses are used to achieve an anaerobic environment in the chamber?
mixture of Nitrogen, Hydrogen and Carbon Dioxide
How many carriers can one wipe treat?
10
Why is the water activity of Renew Life capsules/powder measured?
Water activity can affect shelf life (number of viable cells), texture, taste, color etc
Ranch kills pathogens because it is an_____ food.
Acidified (low pH)
How long is the incubation for a qualitative TB test?
90 days
What does a typical sporicidal carrier look like?
small metal disk
How many carriers must be treated per product in a germicidal spray or wipe test in GLP?
60
What are the two types of media used to enumerate lactics and bifido?
MRS 5.4- lactics
CCMRS- Bifido
Why are the bacteria used in food testing grown in broth media with dextrose?
Acid Adaptation- The bacteria metabolize the dextrose into acid, creating an acidic environment that they adapt to so they are more resistant to acidified ranch
What is this used for in TB testing?
to macerate static M.bovis culture to achieve a homogenous working culture
What are the three parts of the soil typically used in sporicidal testing?
BSA (Bovine Serum Albumin), mucin, yeast extract