Enzymes and Fermentation
Energy Yields of Metabolic Pathways
Requirements for Microbial Growth
Culture techniques and Media
Microbial Growth and Population Dynamics
100

These small RNA molecules act as enzymes and are involved in protein synthesis.

What are ribozymes?

100

The number of ATP molecules directly produced by substrate-level phosphorylation in glycolysis per glucose molecule.

What is 2 ATP?

100

The temperature at which growth occurs fastest is called this.

What is the optimum growth temperature?

100

Organisms that can switch between aerobic respiration and fermentation depending on oxygen availability are classified this way.

What are facultative anaerobes?

100

This is the process by which bacteria increase in number by dividing into two identical daughter cells.

What is binary fission?

200

This part of a holoenzyme is the nonprotein component, which may be a metal ion (inorganic) or an organic molecule.

What is the cofactor?

200

The number of NADH molecules produced in glycolysis per glucose molecule.

What is 2 NADH?

200

These organisms can tolerate high salt concentrations and avoid plasmolysis in hypertonic solutions.

What are halophiles?

200

These media chemically remove molecular oxygen to support growth of anaerobes.

What are reducing media?

200

The phase of bacterial growth in which cells are metabolically active but not dividing rapidly.

What is the lag phase?

300

In this type of phosphorylation, a high-energy phosphate is transferred from an intermediate of catabolism directly to ADP.

What is substrate-level phosphorylation?

300

During the Krebs cycle, the number of NADH molecules produced per glucose molecule.

What is 6 NADH?

300
The three bacterial physical requirements for growth. 

What are temperature, pH, and osmotic pressure?

300

Media that inhibit unwanted organisms and allow growth of desired microbes are called this.

What is selective media?

300

This microbial population phase involves balanced growth where the rate of cell division equals the rate of cell death, often due to nutrient depletion or waste accumulation.

What is the stationary phase?

400

In this type of fermentation, pyruvic acid is reduced by NADH to produce ethanol, and acetaldehyde serves as the final electron acceptor.

What is alcohol fermentation?

400

During the Krebs cycle, the number of FADH₂ molecules produced per glucose molecule.

What is 2 FADH₂?

400

Most bacteria grow best at a pH between these two values.

What are 6.5 and 7.5?

400

This type of culture is used to encourage growth of a particular microorganism in a mixed culture.

What is an enrichment culture?

400

This method of measuring microbial growth counts only cells capable of dividing under specified conditions, and is more accurate than direct microscopic counts for viable cells.

What is the viable plate count (or colony-forming unit) method?

500

This pathway produces one ATP and two NADPH molecules per glucose and is an alternative to glycolysis in some bacteria.

What is the Entner-Doudoroff pathway?

500

The approximate total ATP yield per glucose in aerobic prokaryotes after glycolysis, Krebs cycle, and the electron transport chain, and the total number of NADH and FADH₂ molecules produced.

What is 38 ATP, 10 NADH, and 2 FADH₂?

500

Microbes that require superoxide dismutase and catalase or peroxidase to detoxify oxygen radicals belong to these oxygen-based categories.

What are aerobes, facultative anaerobes, and aerotolerant anaerobes?

500

Microbes can be preserved for long periods either by deep-freezing or this freeze-drying technique.

What is lyophilization?

500

The complex polysaccharide matrix surrounding bacterial cells in biofilms enhances resistance to antibiotics and is synthesized in response to chemical signaling known as this.

What is quorum sensing?