Microbial Control
Growth & Culture
Metabolism & Enzymes
Genetics & DNA
Transport & Nutrition
Microbial Resistance
100

This process destroys all microorganisms, including endospores and viruses.

What is sterilization?

100

This phase of the bacterial growth curve is when cells adjust to a new environment.

What is the lag phase?

100

This molecule is considered the energy currency of the cell.

What is ATP?

100

This enzyme unzips the DNA double helix during replication.

What is helicase?

100

This type of organism uses CO₂ as its carbon source.

What is an autotroph?

100

These structures are among the most resistant forms of microbial life.

What are bacterial endospores?

200

This method is best for sterilizing heat-sensitive liquids.

What is filtration?

200

This phase shows the most rapid cell division.

What is the log (exponential) phase?

200

This stage of respiration produces the most ATP.

What is the electron transport chain?

200

DNA replication is described as this because each new molecule contains one old strand and one new strand.

What is semiconservative replication?

200

This transport mechanism requires ATP to move substances against a gradient.

What is active transport?

200

This infectious agent is even more resistant than endospores.

What are prions?

300

This process reduces microbial numbers to safe public health levels.

What is sanitization?

300

These organisms prefer moderate temperatures around 37°C.

What are mesophiles?

300

This type of inhibition occurs when a molecule binds to a site other than the active site.

What is noncompetitive inhibition?

300

Short DNA fragments formed on the lagging strand are called these.

What are Okazaki fragments?

300

This passive process moves water across a semipermeable membrane.

What is osmosis?

300

High salt or sugar environments inhibit microbes by causing this cellular effect.

What is plasmolysis?

400

These chemicals work by producing reactive oxygen species that damage cells.

What are peroxides?

400

This preservation method removes water from frozen cells for long-term storage.

What is lyophilization?

400

This metabolic process breaks down molecules to release energy.

What is catabolism?

400

This type of mutation results from insertion or deletion of nucleotides.

What is a frameshift mutation?

400

This process uses membrane proteins but does not require energy.

What is facilitated diffusion?

400

These organisms require oxygen to survive.

What are obligate aerobes?

500

This method uses steam under pressure to sterilize equipment.

What is autoclaving?

500

This phase occurs when nutrients become limited and waste accumulates.

What is the stationary phase?

500

This process uses an organic molecule as the final electron acceptor and does not use an electron transport chain.

What is fermentation?

500

This horizontal gene transfer method involves direct cell-to-cell contact using a pilus.

What is conjugation?

500

This type of cell ingestion involves large particles and is sometimes called “cell eating.”

What is phagocytosis?

500

Place these in order from most to least resistant: vegetative cells, prions, endospores.

What are prions → endospores → vegetative cells?